Anti-PECAM1(CD31) Antibody

免疫原 ：0

亚型 ：0

形态 ：0

保存条件 ：0

克隆性 ：单克隆

适应物种 ：human

抗原来源 ：0

库存 ：5-7天

供应商 ：博士德生物

应用范围 ：IHC-P,IC,ELISA

浓度 ：0

规格 ：200ul

产品概况

产品名称	PECAM1(CD31) Antibody
产品描述	Anti-PECAM1(CD31) Antibody validated for IHC, ELISA in Human.
文献引用格式	PECAM1(CD31) Antibody (Boster Biological Technology, Wuhan, China. Catalog #BM1346)
应用范围	IHC-P,IC,ELISA
抗体来源	Mouse
抗体亚型	IgG1
免疫原	Purified recombinant fragment of human CD31 expressed in E. Coli.
抗体规格	200ul/1180元

产品详情

克隆	Monoclonal
克隆号	单克隆抗体：IgG1；克隆号：2F7B2
产品形态	Liquid
内容	Ascitic fluid containing 0.03% sodium azide.
储存条件	连续使用时4°C存储，保质期六个月；长期存储时建议分装为10ul以上小包装-20°C存储，并避免反复冻融，保质期一年。

指标相关信息 (来源: Uniprot.org)

小贴士：你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。

基因名	PECAM1
基因全名	Platelet endothelial cell adhesion molecule
蛋白功能	Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level). .
Tissue Specificity	Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC). .
Sequence Similarities	Platelet endothelial cell adhesion molecule;PECAM-1;EndoCAM;GPIIA';PECA1;CD31;PECAM1;
Uniprot ID	PECAM1: P16284

指标背景简介

CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors.It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. The antibody reacts with the murine form of the Platelet-Endothelial Cell Adhesion Molecule. The reactivity of the antibody is restricted to the isoform of the molecule that is selectively expressed by endothelial cells.The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1.It is also weakly expressed on many peripheral lymphoid cells and platelets.CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).

产品应用细节

为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blotting	1:100-400
Immunohistochemistry in paraffin section	1:50-200
Immunocytochemistry in fixed cells	1:50-200
Flow Cytometry	1:40-80
enzyme-linked immuno sorbent assay	1:2000

*最优稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

产品图片

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[list_product_images]Immunohistochemical analysis of paraffin-embedded human lung cancer (A), lymphonodus tissue (B) and breast cancer (C), showing cytoplasmic localization of vascular endothelial cells using CD31 mouse mAb with DAB staining.|Immunohistochemical analysis of paraffin-embedded human placenta using CD31 mouse mAb.[/list_product_images]