Rat beta IG-H3/TGFBI ELISA Kit

价格： ￥2480

品牌：BOSTER 品牌认证

货号：EK1571

产品详情

产品概况

货号	EK1571
产品名称	Rat beta IG-H3/TGFBI ELISA Kit
产品描述	For quantitative detection of rat beta IG-H3 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
免疫原	NSO, G24-G685
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<15pg/ml
检测范围	156pg/ml-10000pg/ml
基因名称	TGFBI
基因全名	transforming growth factor, beta-induced, 68kDa
Uniprot ID	Q15582
指标别名	Beta ig \| Beta ig h3 \| Beta ig-h3 \| Big h3 \| BIGH3 \| CDB1 \| CDG2 \| CDGG1 \| CSD \| CSD1 \| CSD2 \| CSD3 \| EBMD \| Kerato epithelin \| Kerato-epithelin \| LCD1 \| RGD CAP \| RGD-CAP \| TGFBI \| Q15582
背景资料	Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. It is induced by transforming growth factor-beta and acts to inhibit cell adhesion.
显色时间	15-20分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK1571-CAP	96-well plate precoated with anti- rat beta IG-H3antibody	1
EK1571-ST	Lyophilized recombinant rat beta IG-H3 standard	10ng/tube×2
EK1571-DA	Biotinylated anti- rat beta IG-H3 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。

2.自动洗板机.

3.移液器和枪头。建议使用多通道移液器/排枪。

4.Eppendorf 管。

5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。

◆ 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H₂O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

◆ 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na₂HPO₄ and 0.2g NaH₂PO₄ to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	308	1355	5623	336	1296	5160
Standard deviation	20.63	73.17	314.88	24.19	84.24	309.6
CV(%)	6.7%	5.4%	5.6%	7.2%	6.5%	6%