![SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9]](https://img1.dxycdn.com/2022/0329/869/3916928585916020453-14.jpg)
文献支持SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9]
价格： ￥1700 - 4000
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免疫原 ：The immunogen used to generate this antibody corresponds to SARS-CoV SΔ10 (within S2 domain) protein (1029-1192 a.a.). (SARS-CoV strain: Sin2774) The exact sequence is proprietary.
亚型 ：IgG1
形态 ：Liquid
保存条件 ：Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
克隆性 ：Monoclonal
标记物 ：Unconjugated
适应物种 ：SARS Coronavirus, SARS Coronavirus 2
保质期 ：12 months from the shipping date of the product.
抗原来源 ：SARS Coronavirus
目录编号 ：GTX632604
级别 ：Primary Antibodies
库存 ：Available
供应商 ：GeneTex
宿主 ：Mouse
应用范围 ：WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, EM, Neutralizing/Inhibition, Sandwich ELISA, IHC-P (cell pellet)
浓度 ：1 mg/ml(Please refer to the vial label for the specific concentration.)
靶点 ：Our internal testing indicates no cross-reactivity with MERS-CoV spike protein.This antibody is able to detect multiple SARS-CoV-2 VOCs, including Omicron variant.
抗体英文名 ：SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9]
抗体名 ：SARS-CoV / SARS-CoV-2 (COVID-19) spike 抗体 [1A9]
规格 ：100 μl/25 μl
Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.12.1 variant, His tag (GTX137114-pro) (28.62-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.
SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 (COVID-19) spike protein by immunofluorescent analysis.Sample: BHK-21 cells transfected with full-length SARS-CoV-2 spike were fixed in 4% PFA at RT for 30 min.Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:2000.
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX635672 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 spike (ECD) trimer, omicron BA.2.75 variant, His tag (GTX137533-pro) (28.62-0.45 nM). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (the specified SARS-CoV-2 (COVID-19) Spike antibodies) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Immunoprecipitation of SARS-CoV-2 Spike transfected 293T whole cell extracts using 2 μg of SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604).
Western blot analysis was performed using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604).
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody.
Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, BA.2 / Omicron variant, His tag (GTX137037-pro) (29.71-0.46 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.
Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX135356 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
GeneTex
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入驻年限：12年
