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免疫原 :Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Tubulin 3/ Tuj1. The exact sequence is proprietary.
亚型 :IgG2b
形态 :Liquid
保存条件 :Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性 :Monoclonal
标记物 :Unconjugated
适应物种 :Human, Mouse, Rat, Fish
保质期 :12 months from the shipping date of the product.
抗原来源 :Human
目录编号 :GTX631836
级别 :Primary Antibodies
库存 :Available
供应商 :GeneTex
宿主 :Mouse
应用范围 :WB, ICC/IF, IHC-P, IHC-Fr, IP
浓度 :1 mg/ml (Please refer to the vial label for the specific concentration.)
靶点 :beta III Tubulin antibody [GT11710] specifically recognizes endogenous levels of beta III Tubulin based on the immunogen sequence.
抗体英文名 :beta Tubulin 3/ Tuj1 antibody [GT11710]
抗体名 :beta Tubulin 3/ Tuj1 抗体 [GT11710]
规格 :100 μl/25 μl
beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein expression by immunofluorescent analysis.
Sample: Cultured rat E18 primary hippocampal neuron. Cells were fixed in 4% PFA at RT for 15 min.
Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
beta III Tubulin antibody [GT11710] detects beta III Tubulin proteins on embryonic mouse brain by immunohistochemical analysis.
Sample:Frozen section of embryonic mouse brain (mE18.5).
Red: beta III Tubulin antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: DAPI
beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 rat brain.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX131836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 rat brain.
Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX131836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Mouse tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Immunoprecipitation of beta III Tubulin protein from SK-N-SH whole cell extracts using 5 μg of beta III Tubulin antibody [GT11710] (GTX631836).
Western blot analysis was performed using beta III Tubulin antibody [GT11710] (GTX631836).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at a dilution of 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:2500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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入驻年限:12年