beta Tubulin 3/ Tuj1 antibody [GT11710]

文献支持beta Tubulin 3/ Tuj1 antibody [GT11710]

价格: ¥1700 - 4000

品牌:GeneTex 品牌认证

货号:GTX631836

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免疫原 :Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Tubulin 3/ Tuj1. The exact sequence is proprietary.

亚型 :IgG2b

形态 :Liquid

保存条件 :Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

克隆性 :Monoclonal

标记物 :Unconjugated

适应物种 :Human, Mouse, Rat, Fish

保质期 :12 months from the shipping date of the product. 

抗原来源 :Human

目录编号 :GTX631836

级别 :Primary Antibodies

库存 :Available

供应商 :GeneTex

宿主 :Mouse

应用范围 :WB, ICC/IF, IHC-P, IHC-Fr, IP

浓度 :1 mg/ml (Please refer to the vial label for the specific concentration.)

靶点 :beta III Tubulin antibody [GT11710] specifically recognizes endogenous levels of beta III Tubulin based on the immunogen sequence.

抗体英文名 :beta Tubulin 3/ Tuj1 antibody [GT11710]

抗体名 :beta Tubulin 3/ Tuj1 抗体 [GT11710]

规格 :100 μl/25 μl

beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein expression by immunofluorescent analysis.
Sample: Cultured rat E18 primary hippocampal neuron. Cells were fixed in 4% PFA at RT for 15 min.
Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

beta III Tubulin antibody [GT11710] detects beta III Tubulin proteins on embryonic mouse brain by immunohistochemical analysis.
Sample:Frozen section of embryonic mouse brain (mE18.5).
Red: beta III Tubulin antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: DAPI

beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 rat brain.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX131836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 rat brain.
Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX131836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Mouse tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Immunoprecipitation of beta III Tubulin protein from SK-N-SH whole cell extracts using 5 μg of beta III Tubulin antibody [GT11710] (GTX631836).
Western blot analysis was performed using beta III Tubulin antibody [GT11710] (GTX631836).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at a dilution of 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:2500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

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