![Vimentin antibody [GT812]](https://img1.dxycdn.com/2022/0329/580/7167198865875020453-14.jpg)
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免疫原 ：Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.
亚型 ：IgG2b
形态 ：Liquid
保存条件 ：Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性 ：Monoclonal
标记物 ：Unconjugated
适应物种 ：Human, Mouse, Rat, Cat
保质期 ：12 months from the shipping date of the product.
抗原来源 ：Human
目录编号 ：GTX629744
级别 ：Primary Antibodies
库存 ：Available
供应商 ：GeneTex
宿主 ：Mouse
应用范围 ：WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, Sandwich ELISA
浓度 ：1 mg/ml (Please refer to the vial label for the specific concentration.)
靶点 ：IP/MS validation was supported by references (PMID:30377427)
抗体英文名 ：Vimentin antibody [GT812]
抗体名 ：Vimentin 抗体 [GT812]
规格 ：100 μl/25 μl
Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Vimentin antibody [GT812] detects Vimentin proteins in embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Red: Vimentin antibody [GT812] (GTX629744) diluted at 1:250.
Blue: DAPI.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:75000.
Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629744 as capture antibody at concentration of 5 μg/mL and GTX100619 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:5000.
Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Vimentin antibody [GT812] detects Vimentin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Vimentin protein stained by Vimentin antibody [GT812] (GTX629744) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody (GTX130245) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629744 as capture antibody at concentration of 5 μg/mL and GTX112661 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
Vimentin antibody [GT812] detects Vimentin protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
10 % SDS-PAGE
Vimentin antibody [GT812] (GTX629744) dilution: 1:1000
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT812] (GTX629744) diluted at 1:5000.
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入驻年限：12年
