文献支持AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody
价格： ￥5300
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文献和实验
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免疫原 ：A synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172
亚型 ：IgG
形态 ：Liquid
保存条件 ：Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性 ：Polyclonal
标记物 ：Unconjugated
适应物种 ：Human, Mouse, Rat
保质期 ：12 months from the shipping date of the product.
抗原来源 ：Human
目录编号 ：GTX52341
级别 ：Primary Antibodies
库存 ：Available
供应商 ：GeneTex
宿主 ：Rabbit
应用范围 ：WB, ICC/IF, IHC-P, IHC
浓度 ：1 mg/ml (Please refer to the vial label for the specific concentration.)
靶点 ：The sequence around AMPK alpha 1 (phospho Thr183) is conserved with the sequence around AMPK alpha 2 (phospho Thr172).AAPK1
抗体英文名 ：AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody
抗体名 ：AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) 抗体
规格 ：100 μg
Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody (GTX52341) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
IHC-P analysis of rat spleen tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100
IHC-P analysis of rat skin tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100
IHC-P analysis of human colon tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100
ICC/IF analysis of PFA-fixed C2C12 cells using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Red : Primary antibody
Permeabilization : 0.1% saponin
Dilution : 1:400
WB analysis of PANC1 cells using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Western blot analysis of AMPK alpha phosphorylation expression in Heatshock treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
IHC-P analysis of rat lung tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100
ICC/IF analysis of PFA-fixed 293 cells using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Red : Primary antibody
Permeabilization : 0.1% Triton X-100
Dilution : 1:200
IHC-P analysis of human mammary cancer tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100
IHC-P analysis of human colorectal cancer tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100
IHC-P analysis of human gastric cancer tissue using GTX52341 AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution: 1:100
GeneTex
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入驻年限：12年
