¥1700 - 4000
丁香通研选 科研人放心采购研选PARP antibody [N2C1], Internal
唯一同时通过5大抗体验证的PARP抗体,可进行多种物种及应用实验, WB灵敏度高
产品详情
文献和实验
研选推荐
免疫原 :Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary.
亚型 :IgG
形态 :Liquid
保存条件 :Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性 :Polyclonal
标记物 :Unconjugated
适应物种 :Human, Mouse, Rat
保质期 :12 months from the shipping date of the product.
抗原来源 :Human
目录编号 :GTX112864
级别 :Primary Antibodies
库存 :Available
供应商 :GeneTex
宿主 :Rabbit
应用范围 :WB, ICC/IF, IHC-P, IP, ChIP assay
浓度 :0.28 mg/ml (Please refer to the vial label for the specific concentration.)
靶点 :IP/MS validation was supported by references (PMID:30377409)
抗体英文名 :PARP antibody [N2C1], Internal
抗体名 :PARP 抗体 [N2C1], Internal
规格 :100 μl/25 μl
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-PARP antibody. The precipitated DNA was detected by PCR with primer set targeting to HSP70.1 promoter.
PARP antibody [N2C1], Internal detects PARP protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse spleen.PARP stained by PARP antibody [N2C1], Internal (GTX112864) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 antibody [N2C1], Internal (GTX112864) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PARP1 antibody [N2C1], Internal immunoprecipitates PARP1 protein in IP experiments.
IP samples: HCT-116 whole cell extract
A. 30 μg HCT-116 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of PARP1 protein by 4 μg PARP1 antibody [N2C1], Internal (GTX112864)
5 % SDS-PAGE
The immunoprecipitated PARP1 protein was detected by PARP1 antibody [N2C1], Internal (GTX112864) diluted at 1:3000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
PARP antibody [N2C1], Internal detects PARP protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.PARP stained by PARP antibody [N2C1], Internal (GTX112864) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody [N2C1], Internal (GTX112864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GeneTex
品牌商实名认证
钻石会员
入驻年限:12年