![SOX2 antibody [N1C3]](https://img1.dxycdn.com/2022/0328/220/1538152931904200453-14.jpg)
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免疫原 ：Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.
亚型 ：IgG
形态 ：Liquid
保存条件 ：Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性 ：Polyclonal
标记物 ：Unconjugated
适应物种 ：Human, Mouse, Rat, Frog, Ceratotherium simum
保质期 ：12 months from the shipping date of the product.
抗原来源 ：Human
目录编号 ：GTX101507
级别 ：Primary Antibodies
库存 ：Available
供应商 ：GeneTex
宿主 ：Rabbit
应用范围 ：WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP
浓度 ：0.15 mg/ml (Please refer to the vial label for the specific concentration.)
靶点 ：SOX2
抗体英文名 ：SOX2 antibody [N1C3]
抗体名 ：SOX2 抗体 [N1C3]
规格 ：100 μl/25 μl
SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
Immunofluorescence analysis of PFA-fixed Human ESC, using SOX2(GTX101507) antibody at 1:160 dilution.
SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 rat brain.
Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
SOX2 antibody [N1C3] detects SOX2 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% PFA at RT for 15 min.Green: SOX2 stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 μm.
Flow cytometry on human embryonic stem cells, staining with SOX2 (GTX101507)antibody at 1:100 dilution(purple) or rabbit IgG (black).
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SOX2(GTX101507) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
SOX2 antibody detects SOX2 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101507) at a dilution of 1:2500.
SOX2 antibody [N1C3] detects SOX2 protein at nucleus on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
SOX2 antibody [N1C3] (GTX101507) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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入驻年限：12年
