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免疫原 ：Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary.
亚型 ：IgG
形态 ：Liquid
保存条件 ：Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性 ：Polyclonal
标记物 ：Unconjugated
适应物种 ：Human, Mouse, Rat
保质期 ：12 months from the shipping date of the product.
抗原来源 ：Human
目录编号 ：GTX100042
级别 ：Primary Antibodies
库存 ：Available
供应商 ：GeneTex
宿主 ：Rabbit
应用范围 ：WB, ICC/IF, IHC-P, IHC-Fr, FACS, IHC, IHC (Free Floating)
浓度 ：0.06 mg/ml (Please refer to the vial label for the specific concentration.)
靶点 ：Iba1
抗体英文名 ：Iba1 antibody
抗体名 ：Iba1 抗体
规格 ：100 μl/25 μl
Iba1 antibody detects Iba1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Iba1 antibody detects Iba1 protein at cell membrane by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% PFA at RT for 15 min.Green: Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Correspo
Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Iba1 antibody detects Iba1 protein expression at microglias by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Iba1 protein stained by Iba1 antibody (GTX100042) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Frozen-sectioned mouse brain.Green: Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: ice-cold MeOH for 5 min
Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:1000.
Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Iba1 antibody (GTX100042) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody (GTX100042) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GeneTex
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入驻年限：12年
