库存 :99
供应商 :联科生物
检测限 :62.5 pg/mL - 4000 pg/mL
检测方法 :双抗夹心法
应用 :ELISA
适应物种 :Rat
标记物 :HRP
样本 :血清,血浆,细胞培养上清及其他生物学样本
规格 :10*96T
Rat IL-10 ELISA Kit
描述
商品名 |
Rat IL-10 ELISA Kit (大鼠白细胞介素10 ELISA试剂盒) |
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:3.4% - 5.3%;板间变异系数:4.5% - 6.1% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
20 μL |
灵敏度 |
0.48 pg/mL |
检测范围 |
62.5 pg/mL - 4000 pg/mL |
回收率 |
86% - 108% |
平均回收率 |
0.98 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
- 包被抗IL-10单克隆抗体的96孔聚苯乙烯酶标板
- 大鼠IL-10冻干标准品
- IL-10检测抗体
- 标准品稀释液
- 检测缓冲液(10×)
- 底物(TMB)
- 终止液
- 洗液(20×)
- 封板膜
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-10抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-10与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-10的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长5630 nm)测定吸光度值。
分子信息
概述大鼠 IL10, CSIF, TGIF 靶点信息
IL10 分子靶点信息概述
- 分子名:IL10, interleukin 10
- 基因家族:Interleukins
- 别名:CSIF; TGIF; IL10A; IL-10
- 全称:cytokine synthesis inhibitory factor; T-cell growth inhibitory factor
IL10 分子靶点综述
白细胞介素10(IL-10)是由2个长178个氨基酸的亚基构成的同源二聚体。在人类中,它由位于1号染色体的IL-10基因编码,由5个外显子组成。IL-10主要由单核细胞分泌,淋巴细胞、II型辅助性T细胞(Th2)、肥大细胞、CD4+CD25+Foxp3+调节性T细胞和部分活化的T细胞和B细胞分泌较少。它对免疫调控和炎症具有多种效应。它下调巨噬细胞的Th1细胞因子、II类MHC抗原和共刺激分子。它还能增强B细胞存活、增殖和抗体分泌。IL-10能阻断NF-κB活性,并参与JAK-STAT信号通路调控。IL-10的免疫抑制特性提示其可能应用于临床,抑制器官移植后的排斥。IL-10还具有强烈的消炎特性。
大鼠 Rat Il10 分子靶点信息
- 分子名:Il10, interleukin 10
- 别称:
- CSIF
- cytokine synthesis inhibitory factor
- If2a
- IL-10
- IL10X
- interferon 2a
- interleukin-10
- 基因序列:NCBI_Gene: 25325
- 蛋白序列:
- UniprotKB: A0A7R8C3K4
- UniprotKB: P29456
大鼠 Rat Il10 靶点分子功能(预测)
Enables interleukin-10 receptor binding activity. Involved in several processes, including cellular response to estradiol stimulus; liver regeneration; and negative regulation of nitric oxide biosynthetic process. Located in extracellular space. Used to study several diseases, including lung disease (multiple); perinatal necrotizing enterocolitis; sciatic neuropathy; toxic encephalopathy; and transient cerebral ischemia. Biomarker of several diseases, including artery disease (multiple); decubitus ulcer; lung disease (multiple); obstructive sleep apnea; and postpartum depression. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human IL10 (interleukin 10).
该产品被引用文献
- IF = 18.027 Tailored Apoptotic Vesicle Delivery Platform for Inflammatory Regulation and Tissue Repair to Ameliorate Ischemic Stroke. ACS Nano (2023)
- IF = 15.84 In Situ Regulation of Macrophage Polarization to Enhance Osseointegration Under Diabetic Conditions Using Injectable Silk/Sitagliptin Gel Scaffolds. Advanced Science (2020)
- IF = 14.5 An injectable signal-amplifying device elicits a specific immune response against malignant glioblastoma. Acta Pharmaceutica Sinica B (2023)
- IF = 12.479 Nanofat functionalized injectable super-lubricating microfluidic microspheres for treatment of osteoarthritis. BIOMATERIALS (2022)
- IF = 11.301 Programming electronic skin with diverse skin-like properties. Journal of Materials Chemistry A (2020)
- IF = 11.092 Calcium-Zinc Phosphate Chemical Conversion Coating Facilitates the Osteointegration of Biodegradable Zinc Alloy Implants by Orchestrating Macrophage Phenotype. Advanced Healthcare Materials (2022)
- IF = 10.723 Multifunctional chondroitin sulfate based hydrogels for promoting infected diabetic wounds healing by chemo-photothermal antibacterial and cytokine modulation. CARBOHYDRATE POLYMERS (2023)
- IF = 10.435 Functionalized nanoparticles with monocyte membranes and rapamycin achieve synergistic chemoimmunotherapy for reperfusion-induced injury in ischemic stroke. JOURNAL OF NANOBIOTECHNOLOGY (2021)
- IF = 10.383 Galunisertib-Loaded Gelatin Methacryloyl Hydrogel Microneedle Patch for Cardiac Repair after Myocardial Infarction. ACS Applied Materials & Interfaces (2022)
- IF = 10.383 Multistage ROS-Responsive and Natural Polyphenol-Driven Prodrug Hydrogels for Diabetic Wound Healing. ACS Applied Materials & Interfaces (2022)
- IF = 10.319 Improvement of bone formation by bionic hydroxyapatite nanorod via the regulation of macrophage polarization. JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY (2022)
- IF = 9.229 Polydopamine-Decorated Microcomposites Promote Functional Recovery of an Injured Spinal Cord by Inhibiting Neuroinflammation. ACS Applied Materials & Interfaces (2021)
- IF = 8.986 Dual-Functionalized MSCs that Express CX3CR1 and IL-25 Exhibit Enhanced Therapeutic Effects on Inflammatory Bowel Disease. MOLECULAR THERAPY (2020)
- IF = 8.947 Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury. Acta Biomaterialia (2021)
- IF = 8.469 Hypothermic oxygenated perfusion inhibits HECTD3-mediated TRAF3 polyubiquitination to alleviate DCD liver ischemia-reperfusion injury. Cell Death & Disease (2021)
- IF = 7.561 Probiotic Interventions Alleviate Food Allergy Symptoms Correlated With Cesarean Section: A Murine Model. Frontiers in Immunology (2021)
- IF = 7.446 Discovery and In Vivo Anti-inflammatory Activity Evaluation of a Novel Non-peptidyl Non-covalent Cathepsin C Inhibitor. JOURNAL OF MEDICINAL CHEMISTRY (2021)
- IF = 7.419 Chaihu-Shugan-San inhibits neuroinflammation in the treatment of post-stroke depression through the JAK/STAT3-GSK3β/PTEN/Akt pathway. BIOMEDICINE & PHARMACOTHERAPY (2023)
- IF = 7.129 Probiotics ameliorates pulmonary inflammation via modulating gut microbiota and rectifying Th17/Treg imbalance in a rat model of PM2.5 induced lung injury. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022)
- IF = 6.922 The Anti-Inflammation and Anti-Nociception Effect of Ketoprofen in Rats Could Be Strengthened Through Co-Delivery of a H2S Donor, S-Propargyl-Cysteine. Journal of Inflammation Research (2021)
- IF = 6.706 Reconfiguration of Gut Microbiota and Reprogramming of Liver Metabolism with Phycobiliproteins Bioactive Peptides to Rehabilitate Obese Rats. Nutrients (2022)
- IF = 6.633 Sex-dependent effects of GPER activation on neuroinflammation in a rat model of traumatic brain injury. BRAIN BEHAVIOR AND IMMUNITY (2020)
- IF = 6.419 A novel dendritic mesoporous silica based sustained hydrogen sulfide donor for the alleviation of adjuvant-induced inflammation in rats. DRUG DELIVERY (2021)
- IF = 6.321 The Preparation of a Novel Poly(Lactic Acid)-Based Sustained H2S Releasing Microsphere for Rheumatoid Arthritis Alleviation. Pharmaceutics (2021)
- IF = 6.291 Paraquat promotes acute lung injury in rats by regulating alveolar macrophage polarization through glycolysis. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2021)
- IF = 6.064 Intestinal Microbiota Participates in the Protective Effect of HO-1/BMMSCs on Liver Transplantation With Steatotic Liver Grafts in Rats. Frontiers in Microbiology (2022)
- IF = 6.058 M2 macrophages mediate fibrotic scar formation in the early stages after cerebral ischemia in rats. Neural Regeneration Research (2023)
- IF = 5.811 Metformin Regulates Alveolar Macrophage Polarization to Protect Against Acute Lung Injury in Rats Caused by Paraquat Poisoning. Frontiers in Pharmacology (2022)
- IF = 5.811 Shenling Baizhu Powder Alleviates TNBS-Induced Colitis in Rats by Improving Intestinal Epithelial Permeability and Inhibiting Inflammation Through the TLR5/MyD88/NF-κB Pathway.. Frontiers in Pharmacology (2022)
- IF = 5.811 An Shen Ding Zhi Ling Alleviates Symptoms of Attention Deficit Hyperactivity Disorder via Anti-Inflammatory Effects in Spontaneous Hypertensive Rats. Frontiers in Pharmacology (2021)
- IF = 5.793 Functions of resolvin D1-ALX/FPR2 receptor interaction in the hemoglobin-induced microglial inflammatory response and neuronal injury. Journal of Neuroinflammation (2020)
- IF = 5.7 Macrophage migration inhibitory factor facilitates prostaglandin E2 production of astrocytes to tune inflammatory milieu following spinal cord injury. Journal of Neuroinflammation (2019)
- IF = 5.455 Herbal formula Huangqi Guizhi Wuwu decoction attenuates paclitaxel-related neurotoxicity via inhibition of inflammation and oxidative stress. Chinese Medicine (2021)
- IF = 5.31 Bortezomib alleviates antibody-mediated rejection in kidney transplantation by facilitating Atg5 expression. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021)
- IF = 5.241 Thrombin induces morphological and inflammatory astrocytic responses via activation of PAR1 receptor. Cell Death Discovery (2022)
- IF = 5.2 Astaxanthin prevents osteoarthritis by blocking Rspo2-mediated Wnt/β-catenin signaling in chondrocytes and abolishing Rspo2-related inflammatory factors in macrophages. Aging-US (2023)
- IF = 5.195 Yi-Qi-Jian-Pi formula ameliorates immune function in acute-on-chronic liver failure by upregulating autophagy and mitochondrial biogenesis in CD8+ T lymphocytes. JOURNAL OF ETHNOPHARMACOLOGY (2023)
- IF = 5.195 Xianglian pill modulates gut microbial production of succinate and induces regulatory T cells to alleviate ulcerative colitis in rats. JOURNAL OF ETHNOPHARMACOLOGY (2022)
- IF = 5.194 Safety considerations of chloroquine in the treatment of patients with diabetes and COVID-19. CHEMICO-BIOLOGICAL INTERACTIONS (2022)
- IF = 5.162 A composite hydrogel loading natural polysaccharides derived from Periplaneta americana herbal residue for diabetic wound healing. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)
- IF = 5.093 Protection Against Post-resuscitation Acute Kidney Injury by N-Acetylcysteine via Activation of the Nrf2/HO-1 Pathway. Frontiers in Medicine (2022)
- IF = 4.963 Human amnion-derived mesenchymal stem cells alleviate lung injury induced by white smoke inhalation in rats. Stem Cell Research & Therapy (2018)
- IF = 4.932 Bone marrow mesenchymal stem cells modified with heme oxygenase-1 alleviate rejection of donation after circulatory death liver transplantation by inhibiting dendritic cell maturation in rats. INTERNATIONAL IMMUNOPHARMACOLOGY (2022)
- IF = 4.927 Physicochemical Characteristics and Antidiabetic Properties of the Polysaccharides from Pseudostellaria heterophylla. MOLECULES (2022)
- IF = 4.631 AMPK/SIRT1 Deficiency Drives Adjuvant-Induced Arthritis in Rats by Promoting Glycolysis-Mediated Monocytes Inflammatory Polarization. Journal of Inflammation Research (2022)
- IF = 4.627 Treatment with adipose tissue-derived mesenchymal stem cells exerts anti-diabetic effects, improves long-term complications, and attenuates inflammation in type 2 diabetic rats. Stem Cell Research & Therapy (2019)
- IF = 4.451 Inhibition of Toll-Like Receptor 4 Signaling Pathway Accelerates the Repair of Avascular Necrosis of Femoral Epiphysis through Regulating Macrophage Polarization in Perthes Disease. Tissue Engineering and Regenerative Medicine (2023)
- IF = 4.414 1H NMR Spectroscopy-Based Metabolomics Approach to Study the Anti-Stroke Activity of G-3702, a Novel Better Alternative to DL-3-n-Butylphthalide. NEUROCHEMICAL RESEARCH (2022)
- IF = 4.36 Tibetan medicine Ershiwuwei Lvxue Pill attenuates collagen-induced arthritis via inhibition of JAK2/STAT3 signaling pathway. JOURNAL OF ETHNOPHARMACOLOGY (2021)
- IF = 4.36 Yanning Syrup ameliorates the lipopolysaccharide-induced inflammation: Adjusting the gut microbiota, short-chain fatty acids, and the CD4+ T cell balance. JOURNAL OF ETHNOPHARMACOLOGY (2021)
- IF = 3.943 Hepatocyte growth factor mediates a novel form of hepatic stem/progenitor cell-induced tolerance in a rat xenogeneic liver rejection model. INTERNATIONAL IMMUNOPHARMACOLOGY (2020)
- IF = 3.943 DiR-labeled tolerogenic dendritic cells for targeted imaging in collagen- induced arthritis rats. INTERNATIONAL IMMUNOPHARMACOLOGY (2020)
- IF = 3.69 Ethyl acetate extract of Tibetan medicine Rhamnella gilgitica ameliorated type II collagen-induced arthritis in rats via regulating JAK-STAT signaling pathway. JOURNAL OF ETHNOPHARMACOLOGY (2020)
- IF = 3.647 Selenium/Zinc-Enriched probiotics improve serum enzyme activity, antioxidant ability, inflammatory factors and related gene expression of Wistar rats inflated under heat stress. LIFE SCIENCES (2020)
- IF = 3.575 Vagus nerve stimulation mediates microglia M1/2 polarization via inhibition of TLR4 pathway after ischemic stroke. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2021)
- IF = 3.067 Physiological and histopathological effects of electroporation pulse on stomach of rats. BMC GASTROENTEROLOGY (2021)
- IF = 3.061 The effects of Saccharomyces boulardii on rat colonic hypermotility induced by repeated water avoidance stress and the potential mechanism. PeerJ (2022)
- IF = 2.956 The effect of Astragaloside IV on ethanol-induced gastric mucosal injury in rats: Involvement of inflammation. INTERNATIONAL IMMUNOPHARMACOLOGY (2017)
- IF = 2.935 Paeoniflorin improves cardiac function and decreases adverse postinfarction left ventricular remodeling in a rat model of acute myocardial infarction. Drug Design Development and Therapy (2018)
- IF = 2.928 Carbenoxolone decreases monocrotaline‑induced pulmonary inflammation and pulmonary arteriolar remodeling in rats by decreasing the expression of connexins in T lymphocytes. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2019)
- IF = 2.677 Vagus nerve stimulation promotes the M1-to-M2 transition via inhibition of TLR4/NF-κB in microglial to rescue the reperfusion injury. Journal of Stroke & Cerebrovascular Diseases (2022)
- IF = 2.63 The Effect of a Compound Protein on Wound Healing and Nutritional Status. Evidence-based Complementary and Alternative Medicine (2022)
- IF = 2.63 Xiaoyao-Qingluoyin Cure Adjuvant-Induced Arthritis by Easing LPS Response-Related Pathway-Mediated Immune Abnormality. Evidence-based Complementary and Alternative Medicine (2022)
- IF = 2.359 Ischemic postconditioning ameliorates acute kidney injury induced by limb ischemia/reperfusion via transforming TLR4 and NF-κB signaling in rats. Journal of Orthopaedic Surgery and Research (2021)
- IF = 2.121 Neutrophil extracellular traps contribute to the intestine damage in endotoxemic rats. JOURNAL OF SURGICAL RESEARCH (2014)
- IF = 1.984 Louqin Zhisou Decoction Inhibits Mucus Hypersecretion for Acute Exacerbation of Chronic Obstructive Pulmonary Disease Rats by Suppressing EGFR-PI3K-AKT Signaling Pathway and Restoring Th17/Treg Balance. Evidence-based Complementary and Alternative Medicine (2019)
- IF = 1.984 The Anti-Inflammatory Effect of Feiyangchangweiyan Capsule and Its Main Components on Pelvic Inflammatory Disease in Rats via the Regulation of the NF-κB and BAX/BCL-2 Pathway. Evidence-based Complementary and Alternative Medicine (2019)
- IF = 1.955 Telmisartan ameliorates LPS-induced pneumonia in rats through regulation of the PPARγ/NF-κB pathway. MICROBIOLOGY AND IMMUNOLOGY (2022)
- IF = 1.851 β‑estradiol alleviates hypertension‑ and concanavalin A‑mediated inflammatory responses via modulation of connexins in peripheral blood lymphocytes. Molecular Medicine Reports (2019)
- IF = 1.845 Umbilical Cord Mesenchymal Stem Cells for Inflammatory Regulation After Excision and Grafting of Severe Burn Wounds in Rats. Journal of Burn Care & Research (2021)
- IF = 1.813 The Protective Effects of the Ethyl Acetate Part of Er MiaoSan on Adjuvant Arthritis Rats by Regulating the Function of Bone Marrow-Derived Dendritic Cells. Evidence-based Complementary and Alternative Medicine (2020)
- IF = 1.813 Comparisons of Chemical Profiles and Gastroprotective Effects of Citri Sarcodactylis Fructus Pre- and Poststeam Processing. Evidence-based Complementary and Alternative Medicine (2020)
- IF = 1.786 Zinc-enriched probiotics enhanced growth performance, antioxidant status, immune function, gene expression, and morphological characteristics of Wistar rats raised under high ambient temperature. 3 Biotech (2019)
- IF = 1.41 MicroRNA‑21 inhibits lipopolysaccharide‑induced acute lung injury by targeting nuclear factor‑κB. Experimental and Therapeutic Medicine (2018)
- IF = 1.369 Bovine Lactoferrin Alleviates Pulmonary Lipid Peroxidation and Inflammatory Damage in Heat Stroke Rats. Therapeutic Hypothermia and Temperature Management (2022)
- IF = 1.261 Changes and significance of inflammatory cytokines in a rat model of cervical spondylosis. Experimental and Therapeutic Medicine (2017)
- IF = 0.841 Study on prevention effect of Zishen Yutai pill combined with progesterone for threatened abortion in rats. Asian Pacific Journal of Tropical Medicine (2016)