L-929,小鼠成纤维细胞

细胞类型 ：connective tissue fibroblast

物种来源 ：murine (mouse)

DSMZ no:ACC 2
Species: murine (mouse) (Mus musculus)
Cell type: connective tissue fibroblast
Origin: established from the normal subcutaneous areolar and adipose tissue of a male C3H/An mouse; used as target in TNF detection assays
Biosafety level: 1
Permissions and restrictions: A

DSMZ Cell Culture Data:
Morphology: fibroblasts growing as monolayer
Medium: 90% RPMI 1640 + 10% h.i. FBS
Subculture: split confluent cultures 1:5 to 1:10 using trypsin (do not use trypsin/EDTA); after 2-3 days monolayer will be confluent; split 2-3 times a week; seed out initially at about 1.0-2.0 x 10⁶ cells/25 cm²
Incubation: at 37 °C with 5% CO₂
Doubling time: ca. 21-24 hours
Harvest: ca. 4-8 x 10⁶ cells/25 cm²
Storage: frozen with 70% medium, 20% FBS, 10% DMSO

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