TET2 antibody [N2-2], N-term

文献支持TET2 antibody [N2-2], N-term

价格: ¥1700 - 4000

品牌:GeneTex 品牌认证

货号:GTX124205

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免疫原 :Recombinant protein encompassing a sequence within the N-terminus region of human TET2. The exact sequence is proprietary.

亚型 :IgG

形态 :Liquid

保存条件 :Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

克隆性 :Polyclonal

标记物 :Unconjugated

适应物种 :Human, Mouse

保质期 :12 months from the shipping date of the product. 

抗原来源 :Human

目录编号 :GTX124205

级别 :Primary Antibodies

库存 :Available

供应商 :GeneTex

宿主 :Rabbit

应用范围 :WB, ICC/IF, IHC-P, IP, ChIP assay

浓度 :1.55 mg/ml (Please refer to the vial label for the specific concentration.)

靶点 :TET2

抗体英文名 :TET2 antibody [N2-2], N-term

抗体名 :TET2 抗体 [N2-2], N-term

规格 :100 μl/25 μl

Wild-type (WT) and TET2 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.Green: TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Cross-linked ChIP was performed with U2OS chromatin extract and 5 μg of either control rabbit IgG or anti-TET2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.

TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation of TET2 protein from 293T whole cell extracts using 5 μg of TET2 antibody [N2-2], N-term (GTX124205).
Western blot analysis was performed using TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500.
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

TET2 antibody [N2-2], N-term detects TET2 protein on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded cervical carcinoma.
TET2 antibody [N2-2], N-term (GTX124205) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

TET2 antibody detects TET2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of DDDDK-human TET2-transfected 293T cells
5% SDS-PAGE
TET2 antibody (GTX124205) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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