兔抗光系统I PsaK亚基多克隆抗体

价格： ￥4845

品牌：Agrisera

货号：货号AS04 049

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抗体名：兔抗光系统I PsaK亚基多克隆抗体

抗体英文名：PsaK|PSI-K subunit of photosystem I

浓度：见说明书

应用范围：western blot (WB)

宿主：兔

适应物种：植物

标记物：无

克隆性：多抗/单行

保存条件：低温

亚型：IgG

规格：100 µl

供应商：上海经科化学科技有限公司

服务热线：400-0199-638

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兔抗光系统I PsaK亚基多克隆抗体介绍：

货号：AS04 049

中文名称：兔抗光系统I PsaK亚基多克隆抗体

英文名称：PsaK|PSI-K subunit of photosystem I

应用：western blot (WB)

规格：100 µl

价格：4845元

兔抗光系统I PsaK亚基多克隆抗体简介：

PRODUCT INFORMATION
background		PsaK is a subunit of photosystem I. It has a role in organizing the peripheral light-harvesting complexes on the core antenna of photosystem I. Alternative names: photosystem I subunit X, PSI-K
immunogen		fusion protein between DHFR and the mature part of PsaK from Arabidopsis thaliana Q9SUI5,At1g30380
host		rabbit
clonality		polyclonal
purity		total IgG in PBS pH 7.4,
format		lyophilized
quantity		100 µl
reconstitution		For reconstitution add 100 µl of sterile water.
storage		store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applications		western blot (WB)
related products		antibody collection to PSI proteins
additional information		to be added when available

APPLICATION INFORMATION
recommended dilution		1:2000- 1: 5000 with standard ECL (WB)
expected \| apparent MW		8.5 kDa
confirmed reactivity		Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Spinacia oleracea
predicted reactivity		n.a.
not reactive in		Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942
additional information		not available at the moment
selected references		Bock (2012). The plastid genome-encoded Ycf4 protein functions as a non-essential assembly factor for photosystem I in higher plants. Plant Physiol. ahead of print.

application example

2 µg of total protein from (1) Arabidopsis thaliana leaf extracted with PEB (AS08 300),(2) Horderum vulgare leaf extracted with PEB (AS08 300), (3) Chlamydomonas reinhardtii total cell extracted with PEB (AS08 300), (4) Synechococcus sp. 7942 total cell extracted with PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen)LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Abcam) diluted to 1:20 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).