第一链cDNA合成试剂盒

保存条件 ：Store at -20°C.

英文名 ：RevertAid H Minus First Strand cDNA Synthesis Kit

库存 ：现货

供应商 ：恒斐生物

CAS号 ：相关应用： cDNA 文库和文库构建

规格 ：20 reactions

描述

Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

Highlights

• High yields of full-length first strand cDNA up to 13 kb
• Increased reaction temperatures in the range of 42 to 55°C
• Supplied with the recombinant RiboLock RNase Inhibitor
• Complete kit—oligo(dT)18 and random hexamer primers included with the kit

Applications

• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Construction of full length cDNA libraries
• Antisense RNA synthesis

Additional Features
The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with the reverse transcription reaction, as it maintains activity at temperatures up to 55°C.The kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A), therefore, they can be used for transcription of the 5’-end regions of mRNA or for cDNA synthesis using RNA without the poly(A) tail, e.g., microRNAs. Gene-specific primers may also be used with the kit. The first strand cDNA can be directly used as a template in PCR, realtime PCR or in second strand cDNA synthesis.