Nuclistain核酸染色剂（EB替代品）

价格：询价

品牌：美国Nationaldiagnostics

货号：SG EC-730

产品详情

Nuclistain核酸染色剂（EB替代品）

UV Free Visualization of DNA and RNA
Improves Yield in PCR Purification from Agarose Gels
Easy and Safe

Catalog Number:SG EC-730

1.DNA和RNA检测不需要紫外照射

2.提高PCR产物凝胶回收的完整性和产量

3.核酸阳性染色/高灵敏度

4.安全便捷

Nuclistain核酸染色剂用来替代在单双链DNA/RNA的琼脂糖以及聚丙烯酰胺电泳中使用的EB染色剂，它具有与EB相当的灵敏度，可以检测低至10-50ng的核酸样品。它的优势在于正常的光照条件下可以检测核酸，避免紫外照射对核酸的损伤。另外它的可见光检测大幅度的提高了小于500bp的DNA样品从琼脂糖凝胶中的回收产量。Nuclistain核酸染色剂与核酸的结合是可逆的，不会对核酸进行修饰。Nuclistain核酸染色剂使用起来非常方便，一小瓶25ml的Nuclistain染色剂就可以稀释成2.5L的染色工作液。分离的DNA条带在淡蓝色的背景下显示为深蓝色。

Nuclistain is intended as a replacement for the conventionally used ethidium bromide for the rapid detection of double and single stranded DNA and RNA in agarose and polyacrylamide gels. Its sensitivity is nearly comparable, with the capability of detecting as little as 10-50 ng of nucleic acid sample. The advantage of Nuclistain is that the results are visible under normal lighting conditions, eliminating the possibility of sample damage by UV radiation. Furthermore, Nuclistain visualization dramatically improves the yield of smaller and medium sized DNA (<500 bp) obtained by purification from agarose. Nuclistain binding is reversible and does not modify nucleic acids.

Nuclistain is extremely easy to use. One 25 ml bottle of Nuclistain will make 2.5 liters of stain solution. The separated DNA appears as dark blue bands on a light blue background.

Nuclistain improves the yield of smaller and medium sized DNA (<500 bp) obtained by purification from agarose. One gel was stained with Nuclistain and the other with EtBr. The DNA was subsequently purified from each and then run side by side on a third gel, visualized by EtBr. The results show the improved yield from the Nuclistain.