Oct4 antibody [GT735]

价格： ￥1700 - 4000

品牌：GeneTex 品牌认证

货号：GTX627423

产品详情

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免疫原：Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

亚型：IgG1

形态：Liquid

保存条件：Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

克隆性：Monoclonal

标记物：Unconjugated

适应物种：Human

保质期：12 months from the shipping date of the product.

抗原来源：Human

目录编号：GTX627423

级别：Primary Antibodies

库存：Available

供应商：GeneTex

宿主：Mouse

应用范围：WB, ICC/IF, FACS, IP

浓度：1 mg/ml (Please refer to the vial label for the specific concentration.)

靶点：OCT4

抗体英文名：Oct4 antibody [GT735]

抗体名：Oct4 抗体 [GT735]

规格：100 μl/25 μl

Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX627423) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX627423) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT735] (GTX627423) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Sample (10 ug of whole cell lysate)
A: Non-transfected HeLa lysates
B: OCT 3/4 transfected HeLa lysates
10% SDS PAGE
GTX627423 diluted at 1:5000

Oct4 antibody [GT735] detects Oct4 protein by Western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10 % SDS-PAGE
(GTX627423) dilution: 1:1000

Oct4 antibody [GT735] detects POU5F protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody [GT735] dilution: 1:50

Sample (30 ug of whole cell lysate)
A: NT2D1
10% SDS PAGE
GTX627423 diluted at 1:1000

Oct4 antibody detects Oct4 protein by immunofluorescent analysis.
Sample: human embryonic stem cell were fixed in 4% PFA for 15 min.
Green: Oct4 protein stained by Oct4 antibody (GTX627423) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.