GATA3 antibody

价格： ￥5300

品牌：GeneTex 品牌认证

货号：GTX54527

产品详情

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免疫原：Purified partial recombinant GATA3 protein expressed in E. coli

亚型：IgG

形态：Liquid

保存条件：Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

克隆性：Polyclonal

标记物：Unconjugated

适应物种：Human

保质期：12 months from the shipping date of the product.

目录编号：GTX54527

级别：Primary Antibodies

库存：Available

供应商：GeneTex

宿主：Rabbit

应用范围：WB, ICC/IF, IHC-P, FACS

浓度：1 mg/ml (Please refer to the vial label for the specific concentration.)

靶点：GATA3

抗体英文名：GATA3 antibody

抗体名：GATA3 抗体

规格：100 μg

ICC/IF analysis of MCF7 (left panel) and SH-SY5Y cells (right panel) using GTX54527 GATA3 antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

WB analysis of 15 ug of SH-SY5Y whole cell lysate (WCL), SH-SY5Y nuclear extract (NE) using GTX54527 GATA3 antibody.
Dilution : 1:1000

ICC/IF analysis of MCF-7 cells using GTX54527 GATA3 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.1% Triton„ X-100 for 10 minutes
Dilution : 1 μg/ml in 0.1% BSA incubated for 3 hours at room temperature

WB analysis of whole cell extracts (20 ug lysate) of SH-SY5Y (Lane 1), Jurkat (Lane 2) and K-562 (Lane 3) using GTX54527 GATA3 antibody.
Dilution : 1:500-1:1500

WB analysis of 20 ug of HEK293T cell lysate overexpressing GATA3-DDK (right lane) or empty vector control (left lane) using GTX54527 GATA3 antibody.
Dilution : 1:1000

IHC-P analysis of human tonsil tissue using GTX54527 GATA3 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200