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免疫原 :Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ATG9A. The exact sequence is proprietary.
亚型 :IgG
形态 :Liquid
保存条件 :Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性 :Polyclonal
标记物 :Unconjugated
适应物种 :Human, Mouse, Rat
保质期 :12 months from the shipping date of the product.
抗原来源 :Human
目录编号 :GTX128427
级别 :Primary Antibodies
库存 :Available
供应商 :GeneTex
宿主 :Rabbit
应用范围 :WB, ICC/IF, IHC-P, IP
浓度 :1 mg/ml (Please refer to the vial label for the specific concentration.)
靶点 :ATG9A
抗体英文名 :ATG9A antibody
抗体名 :ATG9A 抗体
规格 :100 μl/25 μl
ATG9A antibody detects ATG9A protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ATG9A stained by ATG9A antibody (GTX128427) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
ATG9A antibody immunoprecipitates ATG9A protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of ATG9A protein by 4 μg ATG9A antibody (GTX128427)
7.5 % SDS-PAGE
The immunoprecipitated ATG9A protein was detected by ATG9A antibody (GTX128427) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
ATG9A antibody detects ATG9A protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG9A antibody (GTX128427) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ATG9A antibody detects ATG9A protein on colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
ATG9A antibody (GTX128427) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
ATG9A antibody detects ATG9A protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 100% MeOH for 5 min.
Green: ATG9A protein stained by ATG9A antibody (GTX128427) diluted at 1:500.
Blue: Hoechst 33342 staining.
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG9A antibody (GTX128427) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ATG9A antibody detects ATG9A protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
7.5% SDS-PAGE
ATG9A antibody (GTX128427) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ATG9A antibody detects ATG9A protein by western blot analysis.
A. 30 μg BCL-1 whole cell lysate/extract
7.5% SDS-PAGE
ATG9A antibody (GTX128427) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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入驻年限:14年