腺病毒包装/ADV/和元生物价格

服务名称：腺病毒|腺病毒包装服务|腺病毒载体|Adenovirus|ADV

提供商：OBiO和元生物

规格：PFU/mL

和元生物践行“赋能基因治疗，共守生命健康”的使命，以核心技术升级、工艺开发升级、成本控制升级，提升基因治疗递送载体的可及性。即日起， AAV包装低至4380元起，慢病毒包装低至2180元起，用实业回报社会、用科技振兴行业，让基因递送触手可及！

腺病毒包装/腺病毒载体/Adenovirus

腺病毒（Adenovirus, ADV）是一种无包膜的线性双链DNA病毒，其空间结构为立体对称的20面体，衣壳由252个壳粒组成，直径在100nm左右。现已知有51种腺病毒，根据组织嗜性不同被分成ABCDEF六个不同亚群，其中C亚群的第2和第5血清型为最常见的腺病毒载体。

ADV特点

●感染能力强 ●快速瞬时表达 ●载体容量大 ●应用范围广 ●肝脏亲嗜性强

感染效果图

ADV体外感染HUVEC DOI: 10.1016/j.cmet.2021.04.006

部分客户文章列表
1. (IF=29.983) Li L, Fu W, Gong X, et al. The role of G protein-coupled receptor kinase 4 in cardiomyocyte injury after myocardial infarction[J]. European Heart Journal, 2021, 42(14): 1415-1430.
2. (IF=27.287) Ji Y X, Wang Y, Li P L, et al. A kinome screen reveals that Nemo-like kinase is a key suppressor of hepatic gluconeogenesis[J]. Cell Metabolism, 2021, 33(6): 1171-1186. e9.
3. (IF=25.617) Fu Z, Zhang X, Zhou X, et al. In vivo self-assembled small RNAs as a new generation of RNAi therapeutics[J]. Cell research, 2021, 31(6): 631-648.
4. (IF=25.617) Fu G B, Huang W J, Zeng M, et al. Expansion and differentiation of human hepatocyte-derived liver progenitor-like cells and their use for the study of hepatotropic pathogens[J]. Cell research, 2019, 29(1): 8-22.
5. (IF=12.27) Zou Y, Wang A, Huang L, et al. Illuminating NAD+ metabolism in live cells and in vivo using a genetically encoded fluorescent sensor[J]. Developmental cell, 2020, 53(2): 240-252. e7.
6. (IF=11.981) Jiang H, Jia D, Zhang B, et al. Exercise improves cardiac function and glucose metabolism in mice with experimental myocardial infarction through inhibiting HDAC4 and upregulating GLUT1 expression[J]. Basic Research in Cardiology, 2020, 115(3): 1-16.
7. (IF=10.717) Zhang Y, Xue W, Zhang W, et al. Histone methyltransferase G9a protects against acute liver injury through GSTP1[J]. Cell Death & Differentiation, 2020, 27(4): 1243-1258.
8. (IF=10.122) Ma X, Zhou Z, Chen Y, et al. RBP4 functions as a hepatokine in the regulation of glucose metabolism by the circadian clock in mice[J]. Diabetologia, 2016, 59(2): 354-362.
9. (IF=9.461) Zhang W, Yang D, Yuan Y, et al. Muscular G9a regulates muscle-liver-fat axis by musclin under overnutrition in female mice[J]. Diabetes, 2020, 69(12): 2642-2654.
10. (IF=8.579) Li Y, Chen M, Zhou Y, et al. NIK links inflammation to hepatic steatosis by suppressing PPARα in alcoholic liver disease[J]. Theranostics, 2020, 10(8): 3579.
11. (IF=8.579) Yu W, Sun S, Xu H, et al. TBC1D15/RAB7-regulated mitochondria-lysosome interaction confers cardioprotection against acute myocardial infarction-induced cardiac injury[J]. Theranostics, 2020, 10(24): 11244.
12. (IF=8.469） Zheng Z, Li Y, Fan S, et al. WW domain-binding protein 2 overexpression prevents diet-induced liver steatosis and insulin resistance through AMPKβ1[J]. Cell death & disease, 2021, 12(3): 1-16.
13. (IF=8.460) Liu D, Tian X, Liu Y, et al. CREG ameliorates the phenotypic switching of cardiac fibroblasts after myocardial infarction via modulation of CDC42[J]. Cell death & disease, 2021, 12(4): 1-16.
14. (IF=6.832) Zheng D, Zhou H, Wang H, et al. Mesenchymal stem cell-derived microvesicles improve intestinal barrier function by restoring mitochondrial dynamic balance in sepsis rats[J]. Stem Cell Research & Therapy, 2021, 12(1): 1-21.
15. (IF=6.831) Shao C, Yang X, Jing Y, et al. The stemness of hepatocytes is maintained by high levels of lipopolysaccharide via YAP1 activation[J]. Stem cell research & therapy, 2021, 12(1): 1-17.

病毒载体比较

AAV
和元生物生产的每一个rAAV都经历数十道工序的锤炼，随机检测的rAAV空壳率<2%、内毒素＜2.5EU/mL；同时，每批rAAV产品在出库前均会进行多轮滴度测定，保证产品的滴度准确，更好地指导实验研究。此外，我们使用大规模GMP级病毒包装质粒，用于研究级rAAV病毒载体的生产，全力减少批次间差异，降低杂质及污染，让基因递送触手可及！

AAV中包总量1.0E+12 v.g., 滴度≥1.0E+12 v.g./mL	病毒包装4380元/个；过表达全套7480元/套；干扰定制（单靶点）5480元/个；干扰全套14000元/套
AAV大包总量5.0E+12 v.g., 滴度≥5.0E+12 v.g./mL	病毒包装6580元/个；过表达全套9680元/套；干扰定制（单靶点）7680元/个
AAV超大包总量1.0E+13 v.g., 滴度≥1.0E+13 v.g./mL	病毒包装8780元/个；过表达全套11880元/套；干扰定制（单靶点）9880元/个
AAV现货光/化学遗传学、钙成像、递质探针、Cre酶	25μL现货：800元/支；50μL现货：1280元/支；100μL现货：2280元/支；

注：1、基因3kb以内，不超载； 2、病毒包装：质粒抽提+病毒包装+滴度检测； 3、过表达全套：基因合成+载体构建+质粒抽提+表达检测+病毒包装+滴度检测+对照病毒（仅限库存对照）； 4、干扰定制（单靶点）：载体构建+质粒抽提+病毒包装+滴度检测+对照病毒（仅限库存对照）。

慢病毒

和元生物生产的每一个慢病毒颗粒都经历数十道工序的锤炼、经历多轮滴度测定，我们使用大规模GMP级病毒包装质粒，用于研究级慢病毒载体的生产，全力减少批次间差异，降低杂质及污染，让基因递送触手可及！

慢病毒中包 1.0E+8TU （基因3kb以内）	过表达全套4580元/套基因合成+载体构建+质粒抽提+病毒包装+滴度检测+对照病毒（仅限库存对照）
	干扰定制（单靶点）2180元/个载体构建+质粒抽提+病毒包装+滴度检测+对照病毒（仅限库存对照）
	干扰全套6480元/套
	载体构建+质粒抽提+病毒包装+滴度检测+对照病毒（仅限库存对照）
	慢病毒包装2580元/个质粒抽提+病毒包装+滴度检测

让基因递送触手可及！
Make Gene Delivery Accessible.

病毒相关技术服务：

病毒包装服务腺相关病毒包装慢病毒包装腺病毒包装逆转录病毒包装

该产品被引用文献

1.     (IF=29.983) Li L, Fu W, Gong X, et al. The role of G protein-coupled receptor kinase 4 in cardiomyocyte injury after myocardial infarction[J]. European Heart Journal, 2021, 42(14): 1415-1430.
2.     (IF=27.287) Ji Y X, Wang Y, Li P L, et al. A kinome screen reveals that Nemo-like kinase is a key suppressor of hepatic gluconeogenesis[J]. Cell Metabolism, 2021, 33(6): 1171-1186. e9.
3.     (IF=25.617) Fu Z, Zhang X, Zhou X, et al. In vivo self-assembled small RNAs as a new generation of RNAi therapeutics[J]. Cell research, 2021, 31(6): 631-648.
4.     (IF=25.617) Fu G B, Huang W J, Zeng M, et al. Expansion and differentiation of human hepatocyte-derived liver progenitor-like cells and their use for the study of hepatotropic pathogens[J]. Cell research, 2019, 29(1): 8-22.
5.     (IF=12.27) Zou Y, Wang A, Huang L, et al. Illuminating NAD+ metabolism in live cells and in vivo using a genetically encoded fluorescent sensor[J]. Developmental cell, 2020, 53(2): 240-252. e7.
6.     (IF=11.981) Jiang H, Jia D, Zhang B, et al. Exercise improves cardiac function and glucose metabolism in mice with experimental myocardial infarction through inhibiting HDAC4 and upregulating GLUT1 expression[J]. Basic Research in Cardiology, 2020, 115(3): 1-16.
7.     (IF=10.717) Zhang Y, Xue W, Zhang W, et al. Histone methyltransferase G9a protects against acute liver injury through GSTP1[J]. Cell Death & Differentiation, 2020, 27(4): 1243-1258.
8.     (IF=10.122) Ma X, Zhou Z, Chen Y, et al. RBP4 functions as a hepatokine in the regulation of glucose metabolism by the circadian clock in mice[J]. Diabetologia, 2016, 59(2): 354-362.
9.     (IF=9.461) Zhang W, Yang D, Yuan Y, et al. Muscular G9a regulates muscle-liver-fat axis by musclin under overnutrition in female mice[J]. Diabetes, 2020, 69(12): 2642-2654.
10.   (IF=8.579) Li Y, Chen M, Zhou Y, et al. NIK links inflammation to hepatic steatosis by suppressing PPARα in alcoholic liver disease[J]. Theranostics, 2020, 10(8): 3579.
11.   (IF=8.579) Yu W, Sun S, Xu H, et al. TBC1D15/RAB7-regulated mitochondria-lysosome interaction confers cardioprotection against acute myocardial infarction-induced cardiac injury[J]. Theranostics, 2020, 10(24): 11244.
12.   (IF=8.469） Zheng Z, Li Y, Fan S, et al. WW domain-binding protein 2 overexpression prevents diet-induced liver steatosis and insulin resistance through AMPKβ1[J]. Cell death & disease, 2021, 12(3): 1-16.
13.   (IF=8.460) Liu D, Tian X, Liu Y, et al. CREG ameliorates the phenotypic switching of cardiac fibroblasts after myocardial infarction via modulation of CDC42[J]. Cell death & disease, 2021, 12(4): 1-16.
14.   (IF=6.832) Zheng D, Zhou H, Wang H, et al. Mesenchymal stem cell-derived microvesicles improve intestinal barrier function by restoring mitochondrial dynamic balance in sepsis rats[J]. Stem Cell Research & Therapy, 2021, 12(1): 1-21.
15.   (IF=6.831) Shao C, Yang X, Jing Y, et al. The stemness of hepatocytes is maintained by high levels of lipopolysaccharide via YAP1 activation[J]. Stem cell research & therapy, 2021, 12(1): 1-17.