LDL is a large protein (MW 3,500 kDa) with a diameter of 25.8 nm.It is composed of approximately 20-25% protein and 75-80% lipid. The lipid portion can be further described as 9% free cholesterol, 42% cholesteryl ester, 20-24% phospholipid, and 5% triglyceride.

Yiyuan biotechnology LDL, is isolated from blood bank produced human plasma, via ultra-centrifugation (1.019-1.063g/cc). It is purified via ultracentrifugationto homogeneity determined by agarose gel electrophoresis.
Our LDL is membrane filtered and aseptically packaged. Store @ 4ºC.

Oxidized Human LDL OxLDL (Cat. No. YB-002)

Our Human LDL is oxidized using Cu2SO4 (oxidant) in PBS. Oxidation is terminated by adding excess EDTA-Na2. Each lot is analyzed on agarose gel electrophoresis for migration versus LDL. This lot of OxLDL migrates 2.0fold further than the native LDL.

Yiyuanbiotech Native-LDL(n-LDL), Acetylated-LDL(Ac-LDL) and Oxidized-LDL(ox-LDL) were loaded on a agarose gel and electrophoresed for 60 minutes. The lipoproteins were stained with Sudan Black(A and B). Oil red O stainning was used to determine the formation of foam cell. RAW264.7 were incubated with with 80μg/mL ox-LDL for 24hrs (C),

High Oxidized LDL (High OxLDL)

Human LDL is oxidized extensively oxidized with Cu2SO4 (oxidant) in PBS at 37°C. It is used to induce cell apoptosis/death and cell injure. More detail about this product is available by sending a e-mail to: yiyuanbiotech@126.com

Acetylated Low Density Lipoprotein(Ac-LDL)

LDL is acetylated with acetic anhydride and dialyzed. It is ultrafiltered through a membrane and packaged aseptically under nitrogen.

DiI-Ox-LDL

oxidized Low Density Lipoprotein, labeled with 1,1’-dioctadecyl – 3,3,3’,3’-tetramethyl-indocarbocyanine perchlorate, which is highly fluorescent lipophilic dyes that diffuse into the hydrophobic portion of the LDL complex without affecting the LDL-specific binding of the apoprotein. DiI-Ox-LDL is used to label both vascular endothelial cells and macrophages. It can be used to identify and/or isolate these cells from mixed cell populations and investigate uptake and binding of modified LDL by different cell types. DiI has been excited at 488nm or 514nm and the emission is detected at 565 nm.

Representive confocal images of suptake of DiI-ox-LDL in macrop-
hages from ApoE knockout mice.

DiI-Ac-LDL

Acetylated Low Density Lipoprotein, labeled with 1,1’-dioctadecyl – 3,3,3’,3’-tetramethyl-indocarbocyanine perchlorate(see DiI-Ox-LDL ). modified low-density lipoprotein (LDL) including Ox-LDL, Ac-LDL can bind to cell membrance via class A scavenger receptor (SR-A) , CD36 and LOX-1 etc. When cells are labeled with DiI-Ac-LDL, the lipoprotein is degraded by lysosomal enzymes and the DiI (fluorescent probe) accumulates in the intracellular membranes. It can be used to quantify uptake of modified LDL. It also be used to identify isolated cells including vascular endothelial cells, macrophages, and endothelial progenitor cells(EPC).

Confocal images indicate
binding of DiI-Ac-LDL in
macrophages membrance

DiI-LDL

Low Density Lipoprotein labeled with 1,1’-dioctadecyl – 3,3,3’,3’-tetramethyl-indocarbocyanine perchlorate(see DiI-Ox-LDL ).

Human High Density Lipoprotein, HDL

Human High Density Lipoprotein, HDL, (Cat. No. YB-003) is isolated from blood bank produced human plasma. It is purified via ultracentrifugation (d=1.063 ～1.21 g/mL) to homogeneity determined by agarose gel electrophoresis.

VLDL

VLDL (d= <1.006 g/mL) is isolated from human plasma by ultracentrifugation. The purified VLDL is ultrafiltered through a membrane and packaged aseptically under nitrogen.

anti-SRA and anti-CD36

The macrophage class A scavenger receptor (SR-A) and CD36 mediate the uptake of modified low-density lipoprotein (LDL) including Ox-LDL, Ac-LDL, anti-SR-A and anti-CD36 is an affinity purified goat polyclonal antibody raised against a peptide mapping within an internal region of SR-A of human origin.

Western blot analysis of SR-A
expression in THP-1 whole cell
lysate

试剂盒

CCK-8细胞增殖/存活试剂盒(CCK-8 kit)

BCA 蛋白定量试剂盒

油红O染色试剂盒(Oil Red O Staining kits)

小鼠腹腔巨噬细胞分离试剂盒

CCK-8细胞增殖/存活试剂盒(CCK-8 kit)

试剂盒反应原理简介：

Cell Counting Kit-8（CCK-8试剂盒）是检测细胞增殖、细胞存活和细胞毒性的试剂盒，是一种基于水溶性四唑盐的广泛应用快速高灵敏度检测试剂盒，为MTT法的替代方法，试剂盒中采用水溶性四唑盐在电子耦合试剂存在的情况下，可以被线粒体内的一些脱氢酶还原生成橙黄色的formazan(参考图1)。细胞增殖越多越快，则颜色越深；细胞毒性越大，则颜色越浅。对于同样的细胞，颜色的深浅和细胞数目呈良好线性关系。

CCK-8是MTT的一种升级产品，和MTT或其它MTT类似产品如XTT、MTS等相比有明显的优点。第一，MTT被线粒体内的一些脱氢酶还原生成的formazan不是水溶性的，需要有特定的溶解液(如DMSO等)来溶解；而CCK-8和XTT、MTS产生的formazan都是水溶性的，可以省去后续的溶解步骤，减少误差。其次，CCK-8产生的formazan溶解度远远大于XTT和MTS产生的formazan。第三，CCK-8比XTT和MTS更加稳定，使实验结果更加稳定。另外，CCK-8和MTT、XTT等相比线性范围更宽，灵敏度更高。Yuanyuanbiotech研制的CCK-8试剂盒全部采用高纯原装进口原料，经过至少35种细胞检测其OD值与细胞数目的线性关系(r2>0.98)且无细胞毒性。

油红O染色试剂盒(Oil Red O Staining kits)

Atherosclerotic lesions were measured by lipid deposition stained YB oil red O kit represented here in red within thoraco-abdominal aortas from ApoE mice without(upper aortas) and with(lower aortas) high lipid dietand with.

BCA 蛋白定量试剂盒

产品特点：

增强型BCA蛋白浓度测定试剂盒(Enhanced BCA Protein Assay Kit)是根据目前世界上最常用的两种蛋白浓度检测方法之一BCA法改进研制而成，实现了蛋白浓度测定的简单、高稳定性、高灵敏度和高兼容性。

灵敏度高，检测浓度下限达到10μg/ml，最小检测蛋白量达到0.2μg，待测样品体积为1-25μl。

在20-2000μg/ml浓度范围内有较好的线性关系（如图1）。

图1、用该试剂盒检测0.5-10mg/ml不同浓度
BSA 标准曲线，R2>0.99.

BCA法测定蛋白浓度不受绝大部分样品中的化学物质的影响，可以兼容样品中高达5%的SDS，5%的Triton X-100，5%的Tween 20, 60, 80。但受螯合剂和略高浓度的还原剂的影响，需确保EDTA低于10mM，无EGTA，二硫苏糖醇低于1mM，β-巯基乙醇低于0.01%。不适用BCA法。

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E-mail: yuanyuanbiotech@126.com 或 yiyuanbiotech@126.com