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库存 :大量供应
供应商 :康朗生物
检测限 :详见说明书
检测方法 :夹心法
应用 :ELISA 定量检测
标记物 :详见说明书
样本 :血清,血浆,尿液 细胞裂解液,组织匀浆,细胞培养上清液
规格 :96T/盒
Rabbit Von Willebrand Factor (vWF) ELISA Kit
Product name: | Rabbit Von Willebrand Factor (vWF) ELISA Kit |
Method: | Competitive |
Synonyms: | |
Catalog number: | DL-vWF-Rb |
Detection range: | 12.35-1,000ng/mL |
Size: | 96T |
Assay length | 1-4.5Hours |
Price: | inquiry |
Quality guarantee period: | for 12 months |
Introduction
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to this index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled the index and unlabeled this index (Standards or samples) with the pre-coated antibody specific to the index. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of the index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the index in the sample.
Recovery
Matrices listed below were spiked with certain level of recombinant the index and the recovery humanes were calculated by comparing the measured value to the expected amount of the index in samples.
The linearity of the kit was assayed by testing samples spiked with appropriate concenthumanion of the index and their serial dilutions. The results were demonsthumaned by the percentage of calculated concenthumanion to the expected.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50µL Stop Solution. Read at 450 nm immediately
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DL-VLDL-Mu 小鼠极低密度脂蛋白(VLDL) ELISA Kit mouse Very Low Density Lipoprotein (VLDL) ELISA Kit Mus musculus mouse 18.75-1200ng/mL 5.7ng/mL 96T 3800 12 months
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Rabbit Von Willebrand Factor (vWF) ELISA Kit
Item | Standard | Test Result | |
Description | This immunoassay kit allows for the specific measurement of this index in serum, Plasma , Urine ,tissue homogenates and Cell culture supernates and Other biological fluids.. |
Conform | |
Identification | Colorimetric | Positive | |
Composition | Pre-coated, ready to use 96-well strip plate Standard (freeze dried) Standard Diluent Detection Reagent A Detection Reagent B Assay Diluent A Assay Diluent B TMB Substhumane Stop Solution Wash Buffer(30 x concenthumane) Plate sealer for 96 wells Instruction manual |
1 2 1 × 20ml 1× 120μl 1× 120μl 1 × 12ml 1 × 12ml 1 × 9ml 1 ×6ml 1 ×20ml 2 1 |
Conform |
Rabbit Von Willebrand Factor (vWF) ELISA Kit
Test principleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to this index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled the index and unlabeled this index (Standards or samples) with the pre-coated antibody specific to the index. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of the index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the index in the sample.
Recovery
Matrices listed below were spiked with certain level of recombinant the index and the recovery humanes were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 81-93 | 86 |
EDTA plasma(n=5) | 80-97 | 88 |
heparin plasma(n=5) | 90-101 | 95 |
Rabbit Von Willebrand Factor (vWF) ELISA Kit
LinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concenthumanion of the index and their serial dilutions. The results were demonsthumaned by the percentage of calculated concenthumanion to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 82-96% | 83-98% | 81-99% | 93-101% |
EDTA plasma(n=5) | 88-101% | 86-95% | 90-102% | 80-93% |
heparin plasma(n=5) | 80-91% | 82-90% | 95-104% | 79-95% |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50µL Stop Solution. Read at 450 nm immediately
DL-PCDH15-Mu 小鼠原钙黏素15(PCDH15) ELISA Kit mouse Protocadherin 15 (PCDH15) ELISA Kit Mus musculus mouse 0.156-10ng/mL 0.076ng/mL 96T 3980 12 months
DL-RAD51-Hu 人RAD51同源物(RAD51) ELISA Kit human RAD51 Homolog (RAD51) ELISA Kit Homo sapiens human 0.313-20ng/mL 0.081ng/mL 96T 4230 12 months
DL-MIA1-Hu 人黑素瘤抑制性活性蛋白1(MIA1) ELISA Kit human Melanoma Inhibitory Activity Protein 1 (MIA1) ELISA Kit Homo sapiens human 7.813-500pg/mL 2.5pg/mL 96T 3880 12 months
DL-VLDL-Mu 小鼠极低密度脂蛋白(VLDL) ELISA Kit mouse Very Low Density Lipoprotein (VLDL) ELISA Kit Mus musculus mouse 18.75-1200ng/mL 5.7ng/mL 96T 3800 12 months
DL-COMT-Hu 人儿茶酚-O-甲基转移酶(COMT) ELISA Kit human Catechol-O-Methyltransferase (COMT) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.058ng/mL 96T 3880 12 months
DL-PST-Hu 人苯酚磺基转移酶(PST) ELISA Kit human Phenol Sulfotransferase (PST) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.064ng/mL 96T 3705 12 months
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