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免疫原 :Recombinant protein encompassing a sequence within the N-terminus region of human beta Catenin. The exact sequence is proprietary.
亚型 :IgG
形态 :Liquid
保存条件 :Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性 :Polyclonal
标记物 :Unconjugated
适应物种 :Human, Mouse, Rat, Zebrafish, Rabbit, Cat, Dog
保质期 :12 months from the shipping date of the product.
抗原来源 :Human
目录编号 :GTX101435
级别 :Primary Antibodies
库存 :Available
供应商 :GeneTex
宿主 :Rabbit
应用范围 :WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, FACS, IP, ChIP assay, IHC
浓度 :0.07 mg/ml (Please refer to the vial label for the specific concentration.)
靶点 :beta Catenin
抗体英文名 :beta Catenin antibody [N1N2-2], N-term
抗体名 :beta Catenin 抗体 [N1N2-2], N-term
规格 :100 μl/25 μl
Wild-type (WT) and beta Catenin knockout (KO) 293T cell extracts (9 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500.
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500.
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:1000 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in human cervix by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Confocal immunofluorescence analysis (Olympus FV10i) of PFA-fixed A431, using beta Catenin(GTX101435) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at cell membrane and cytoplasm in human esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus.
beta Catenin antibody [N1N2-2], N-term (GTX101435) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
beta Catenin antibody [N1N2-2], N-term (GTX101435) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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入驻年限:12年