Advanced 1step Matermix / 一步法RT-qPCR试剂盒（染料法）价格

英文名：ADVANCEDtm qPCR 1 step mastermix withSUPERGREENtm dye

供应商：wisent维森特

规格：100 reactions

Ordering Information:

Catalogue number	Quantity	Specification
801-001-AR	100 reactions	LOW ROX
801-001-CR	500 reactions	LOW ROX
801-001-LR	1200 reactions	LOW ROX

801-002-AR	100 reactions	HIGH ROX
801-002-CR	500 reactions	HIGH ROX
801-002-LR	1200 reactions	HIGH ROX

For instrument compatibility refer to APPENDIX I
Product Description:
WISENT ADVANCED 1-Step SUPERGREEN mix is used to quantify any RNA template including mRNA, total RNA and viral sequences. Our mix allows low copy number targets to be detected specifically with high efficiency. Proprietary small molecular inhibitor technology prevents formation of primer dimers leading to improved reaction sensitivity and specificity. Advanced buffer chemistry and polymerase technology offer market leading performance with minimal or no optimisation. Our mix is also ideal for fast and ultra fast cycling conditions.
Advanced technology for efficient cDNA synthesis and qPCR in a single tube features:
Thermostable reverse transcriptase 45°C to 55°C
Advanced RNase inhibitor
SUPERGREEN intercalating dye
Rapid extension rate for early Ct values
Best available sensitivity - increased limit of detection
Ideal on all real-time PCR platforms - standard and fast cycling conditions
Product Stability And Storage:
On arrival store at -20°C.
Avoid prolonged exposure to light.
If stored correctly the mastermix will retain full activity for 12 months. It is stable at 4°C for 1 month and can go through 30 freeze/thaw cycles with no loss of activity.
Instructions For Use:
Note: For efficient amplification under fast cycling conditions we recommend amplicon lengths between 80bp and 200bp. Do not exceed 400 bp. The shorter the amplicon length the faster the reaction can be cycled. Primers should have a predicted melting temperature of around 60°C, using default Primer 3 settings.
Reaction Setup:
1. Before starting, briefly vortex 2x WISENT ADVANCED 1 step mix .
2.Prepare a final mix , recommended 20 ul, as follows

Add	Volume	Final concentration
WISENT ADVANCED 1 step mix	10 ul	1X
Forward primer (10 uM)	0.8 ul	400 nM
Reverse primer (10 uM)	0.8 ul	400 nM
20 x RTase	1 -2 ul	1.0 ul is recommended . 2.0 ulmight increase dimer primers
Template RNA	total RNA :1 pg to 1 ug mRNA : >0.01 pg	variable
Add PCR grade water up to 20 ul final volume

3. Program your instrument using following conditions, acquiring data on the SYBR® Green or FAM channel:

Cycles	Temperature	Time	Notes
1	45 to 55 °C	10 min	45 °C:Recommended for most applications , 55 °C to be used only if amplicon contains region of high secondary structure
	95°C	2 minutes	Polymerase activation
	95°C	5 seconds	Denaturation
	60°C to 65°C	20-30 seconds	Anneal/Ｅxtension do not exceed 30 seconds ; do not use bellow 60 °C
Melt analysis	Refer to instrument instructions		optional

APPENDIX I
HIGH ROX / LOW ROX INSTRUMENT COMPATIBILITY
FOR FOLLOWING KITS:
-WISENT ADVANCEDtm qPCR MASTERMIX WITH SUPERGREENtm DYE
-WISENT ADVANCEDtm qPCR 1 step mastermix with SUPERGREENtm dye
-WISENT ADVANCEDtm qPCR PROBE 1 step mastermix
-WISENT ADVANCEDtm qPCR GENOTYPING mastermix

Manufacturer	Instrument	Lo-ROX	Hi ROX
Analytica Jena	qTower	Yes	Yes
Applied Biosystems	7500, 7500 FAST, Viia7TM	Yes	No
Applied Biosystems	7000, 7300,7700,7900, 7900HT, 7900HT FAST, StepOneTM, StepOneTM Plus	No	Yes
Bio-Rad®	iCycler®, MyiQ®, iQ TM5, OpticonTM, OpticonTM2, Chromo4TM, MiniOpticonTM, CFX96TM, CFX384TM	Yes	No
Cepheid®	Smartcycler®	Yes	Yes
Eppendorf	Mastercycler® ep realplex, Mastercycler® realplex 2S	Yes	Yes
Illumina®	EcoTM	Yes	Yes
Qiagen/Corbett	Rotor-GeneTM 3000, 6000, Q	Yes	Yes
Roche Applied Science	Lightcycler®480, Lightcycler®Nano	Yes	Yes
Stratagene (Agilent)	MX 4000P®, MX 3000P®, MX 3005P®	Yes	No
Takara	Cycler Dice®	Yes	Yes
Techne	Quantica®	Yes	Yes
Manufacturer	Instrument	Lo-ROX	Hi ROX