库存 ：现货
检测限 ：4.4 ng/ml - 50 ng/ml
检测方法 ：Colorimetric
应用 ：Sandwich (quantitative)
标记物 ：人CD31 ELISA试剂盒(PECAM) SimpleStep
样本 ：Serum, Cell culture extracts, Adherent cells, Suspension cells, Heparin Plasma, EDTA Plasma, Citrate Plasma
规格 ：96t
- 产品名称人CD31 ELISA试剂盒(PECAM) SimpleStep
- 检测方法Colorimetric
- 精确度
批次内
| 样品 |
n |
Mean |
SD |
CV% |
| Jurkat |
8 |
|
|
6.3% |
- 样品类型
Serum, Cell culture extracts, Adherent cells, Suspension cells, Heparin Plasma, EDTA Plasma, Citrate Plasma
- 检测类型Sandwich (quantitative)
- 灵敏度
1.3 ng/ml
- 范围
4.4 ng/ml - 50 ng/ml
- 复检
特定样本复检结果
| 样品类型 |
平均% |
范围 |
| Serum |
110 |
99% - 119% |
| Cell culture media |
93 |
89% - 97% |
| Heparin Plasma |
97 |
94% - 100% |
| EDTA Plasma |
100 |
89% - 110% |
| Citrate Plasma |
84 |
82% - 86% |
- 检测时间
1h 30m
- 实验步骤One step assay
- 种属反应性
与反应: Human
不与反应: Mouse, Rat, Rabbit, Goat, Chicken, Guinea pig, Hamster, Cow, Dog
- 产品概述
Abcam’s CD31 (PECAM1) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD31 protein in Human serum, plasma, cell culture supernatants and cell extracts.
The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Sensitivity:
Samples diluted in 1X Cell Extraction Buffer PTR – 1.3 ng/mL
Samples diluted in Sample Diluent NS – 2.2 ng/mL
- 说明
CD31 (PECAM1) is a 130-kDa glycoprotein constitutively expressed on endothelial cells, platelets, neutrophils, monocytes, lymphocytes and basophils. CD31 molecules is expressed in large amounts in the intracellular junctions of endothelial cells. Experiments using antibody results in blocking CD31 (PECAM1) homophilic interaction and inhibition of leukocytes transmigration. Expression of CD31 (PECAM1) on the cell surface is regulated by receptor shedding resulting in generation of a soluble 100-kDa extracellular domain form and a truncated 28-kDa fragment containing the transmembrane and cytoplasmic domain of CD31 (PECAM1).
- 经测试应用Sandwich ELISA
- 平台Microplate (12 x 8 well strips)
- 存放说明Store at +4°C. Please refer to protocols.
-
| 组件 |
1 x 96 tests |
| 10X CD31 Capture Antibody |
1 x 600µl |
| 10X CD31 Detector Antibody |
1 x 600µl |
| 10X Wash Buffer PT (ab206977) |
1 x 20ml |
| 50X Cell Extraction Enhancer Solution (ab193971) |
1 x 1ml |
| 5X Cell Extraction Buffer PTR (ab193970) |
1 x 10ml |
| Antibody Diluent 5BI |
1 x 6ml |
| CD31 Human Lyophilized Recombinant Protein |
2 vials |
| Plate Seals |
1 unit |
| Sample Diluent NS |
1 x 50ml |
| SimpleStep Pre-Coated 96-Well Microplate |
1 unit |
| Stop Solution |
1 x 12ml |
| TMB Substrate |
1 x 12ml |
