库存 ：现货
检测限 ：31 ng/ml - 2000 ng/ml
检测方法 ：Colorimetric
应用 ：Sandwich (quantitative)
标记物 ：人alpha 1 Antitrypsin ELISA试剂盒(SERPINA1) SimpleStep
样本 ：Serum, Heparin Plasma, Citrate Plasma
规格 ：96t
- 产品名称人alpha 1 Antitrypsin ELISA试剂盒(SERPINA1) SimpleStep
- 检测方法Colorimetric
- 精确度
批次内
| 样品 |
n |
Mean |
SD |
CV% |
| Overall |
5 |
|
|
3.9% |
批次间
| 样品 |
n |
Mean |
SD |
CV% |
| Overall |
3 |
|
|
2.1% |
- 样品类型
Serum, Heparin Plasma, Citrate Plasma
- 检测类型Sandwich (quantitative)
- 灵敏度
13 ng/ml
- 范围
31 ng/ml - 2000 ng/ml
- 复检
特��样本复检结果
| 样品类型 |
平均% |
范围 |
| Serum |
96 |
92% - 101% |
| Heparin Plasma |
99 |
94% - 102% |
| EDTA Plasma |
77 |
70% - 87% |
| Citrate Plasma |
85 |
83% - 86% |
- 检测时间
1h 30m
- 实验步骤One step assay
- 种属反应性
与反应: Human
- 产品概述
Abcam’s alpha 1 Antitrypsin (SERPINA1) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 1 Antitrypsin protein in Human serum and plasma samples.
The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
- 说明
alpha 1 Antitrypsin (SERPINA1) is a serine protease inhibitor found in a variety of biological fluids. While it can inhibit trypsin, chymotrypsin, and plasminogen activator, it’s main target is elastase. Alpha 1 antitrypsin plays a critical role in the lung by inhibiting the activity of elastase, produced by neutrophils during inflammatory responses. The aberrant form plays a role in coagulation and insulin induced NO synthesis. Alpha 1 antitrypsin deficiency results in emphysema and may also result in liver disease.
- 经测试应用Sandwich ELISA
- 平台Microplate
性能
- 存放说明Store at +4°C. Please refer to protocols.
-
| 组件 |
1 x 96 tests |
| 10X alpha 1 Antitrypsin Capture Antibody |
2 x 300µl |
| 10X alpha 1 Antitrypsin Detector Antibody |
2 x 300µl |
| 10X Wash Buffer PT (ab206977) |
1 x 20ml |
| 50X Cell Extraction Enhancer Solution (ab193971) |
1 x 1ml |
| 5X Cell Extraction Buffer PTR (ab193970) |
1 x 10ml |
| alpha 1 Antitrypsin Human Lyophilized Purified Protein |
2 vials |
| Antibody Diluent 4BI |
2 x 3ml |
| Plate Seals |
1 unit |
| Sample Diluent NS (ab193972) |
1 x 12ml |
| SimpleStep Pre-Coated 96-Well Microplate |
1 unit |
| Stop Solution |
1 x 12ml |
| TMB Substrate |
1 x 12ml |
