His-Tag Antibody

Product Description

This high affinity His-tag antibody is developed using a polyhistidine peptide (HHHHHH) conjugated to KLH and affinity-purified with the HHHHHH peptide on agarose. The antibody is useful for detection, affinity isolation, enrichment and localization of His-tag proteins.

Western-blot with His-tag antibody, HRP (ICP0851) of the His-tag protein (50 kDa) expressed cell lysate and enriched His-tag protein by IP with anti-His-tag (ICP0850) 1: Molecular marker 2: 10 µL of His-tag protein in lysate (original cell lysate) 3: 10 µL of His-tag protein in lysate (after IP) 4: 10 µL of the His-tag protein in 100 µL of the IP eluting buffer, enriched with 25 µg of the anti-His tag (ICP0850) from 2 mL of the original lysate

Species

Rabbit

Formulation

250 µg/mL in PBS, 50% glycerol

Immunogen

Polyhistidine peptide (HHHHHH) KLH conjugates

Purification

Antigen-specific affinity chromatography

Specificity

The antibody recognizes His-tag proteins.

Applications

ELISA (0.25-1 µg/mL); IP ( 1-10 µg, based on the sample size) ; WB (0.25-1.0 µg/mL); IHC (5 µg/mL)

Scientific Description

Polyhistidine peptide is frequently used as an affinity tag to facilitate the isolation of the 6H-fusion proteins using a metal-chelate matrix.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is three years from date of shipping if stored properly.