HA Antibody

Product Description

This high affinity anti-HA antibody is developed using HA peptide (YPYDVPDYA) conjugated to KLH and affinity-purified with the HA peptide. The antibody is useful for detection, isolation and localization of the HA-tag proteins.

Western-blot analysis of the HA-tag protein (50 kd) expressed cell lysate and affinity enriched by IP, with HA-tag antibody, HRP conjugates (ICP0751) 1: Molecular marker 2: 10 uL of HA-tag protein in lysate (original cell lysate) 3: 10 uL of His-tag protein in lysate (after IP depletion) 4: 10 uL of the HA-tag protein in 100 uL of the eluting buffer enriched with 25 ug of the anti-His tag (ICP0750) from 2 mL of the original lysate.

Western blot analysis of the cell extract with expression of HA-tag proteins (B-F) and without expression of HA (A).

Species

Rabbit

Formulation

500 µg/mL in PBS, 50% glycerol

Immunogen

GST peptide EEHLYERDEGDKWR KLH conjugates

Purification

Antigen-specific affinity chromatography

Specificity

The antibody recognizes HA-tag proteins.

Applications

ELISA (1:2500); WB (1:1000); IP (5 µg/sample)

Scientific Description

Hemaglutinin peptide (HA) is frequently used as an affinity tag to facilitate the detection, isolation and localization of the HA-fusion proteins.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C.Expiration date is three years from date of shipping if stored properly.

References

1. Cancer Research, December 15, 2010, 70;10445.

doi: 10.1158/0008-5472.CAN-10-3040