Phospho-Threonine Antibody

Product Description

Affinity purified rabbit polyclonal phospho-threonine antibody (anti-pT) was developed using a phospho-threonine-KLH conjugate and phosvitin mixture as the immunogen. The phospho-threonine specific antibody was affinity purified with phospho-threonine immobilized on agarose.

Detection of a phosphorylated protein kinase with anti-phosphothreonine (ICP9807) Lane1-2: Untreated kinase Lane 3-4: Kinase treated with phosphatase PP1G

Western blot analysis of mouse extract spleen with ICP9807 A: Extract of spleen from mouse treated with sodium vanadate B: Extract of spleen from control mouse

Species

Rabbit

Formulation

250 µg/mL in PBS, pH 7, with 50% glycerol

Immunogen

Phospho-threonine KLH conjugates and phosvitin mixture

Purification

The antibody was affinity purified with phospho-threonine on agarose followed by affinity absorption with threonine on agarose.

Specificity

The antibody recognizes proteins and peptides phosphorylated on threonine residues. There are no cross reactions with phospho-tyrosine.

Species Tested

Human and mouse

Applications

ELISA; WB; IP

Scientific Description

Threonine is one of the 20 naturally occurring amino acids. Kinases phosphorylate threonine to phospho-threonine which enters various biosynthetic pathways in the body.

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.

References

1. M.J. Betts, R.B. Russell. Bioinformatics for Geneticists, M.R. Barnes, I.C. Gray eds, Wiley, 2003.