AmpliTaq Gold® DNA Polymerase with Buffer II and MgCl2价格_品牌:Applied Biosystems-丁香通官网

保存条件：Store at -15 to -30°C.

英文名：DNA Polymerase with Buffer II and MgCl2

库存：现货供应

CAS号：分子生物学酶 › PCR 酶和试剂盒 ›

规格：250 units

货号	单位规格	目录价格 (CNY)	针对您的报价 (CNY)	库存状态
N8080241	250 units	2,745.00	2,745.00	现货供应
N8080243	1500 units	14,380.00	14,380.00	缺货
N8080245	3,000 units	25,550.00	25,550.00	现货供应
N8080247	1000 units	9,790.00	9,790.00	现货供应
N8080249	5,000 units	39,340.00	39,340.00	现货供应
N8080259	25 x 1,000 units	159,400.00	159,400.00	缺货

描述

AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase requiring thermal activation. Features of this enzyme:

• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp® 10X PCR Buffer II with MgCl2

Hot-start activation
The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

Higher specificity, higher yield
AmpliTaq Gold® DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold® DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.

AmpliTaq Gold® 360 DNA Polymerase for superior performance
Also available is AmpliTaq Gold® 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.

规格

Polymerase:	AmpliTaq Gold® DNA Polymerase
Hot Start:	Built-In Hot Start
Fidelity (vs. Taq):	1 X
GC-Rich PCR Performance:	Low
Reaction Format:	Separate Components
Product Overhang:	3'-A
Reaction Speed:	Standard
Final Product Size(s):	5 kb or less
Product Size:	250 units
Exonuclease Activity:	5' - 3'
Shipping Condition:	Dry Ice

内容及储存

Contents: 50 µl of AmpliTaq Gold® DNA Polymerase (at 5 U/µl, 250 units total), 1.5 mL of Buffer II (100 mM Tris-HCl, pH 8.3, 500 mM KCl), and 1.5 mL magnesium chloride Solution.

Store at -15 to -30°C.