人抗流行性出血热病毒抗体IgG（EHF）检测试剂盒

库存 ：563

供应商 ：齐一生物科技（上海）有限公司

检测限 ：详细请见说明书

检测方法 ：ELISA酶联免疫法

应用 ：科研检测

标记物 ：见说明

样本 ：血清，血浆，尿液，胸腹水，脑脊液，细胞培养上清，组织匀浆等

规格 ：48T/96T

人抗流行性出血热病毒抗体IgG（EHF）检测试剂盒齐一生物科技（上海）有限公司产品已被广泛应用于化学、化工、生命科学的基础研究和开发应用、制药、疾病诊断与控制、人口与健康、生物技术等诸多领域.客户遍布国内各大学、研究所、医院、卫生防疫、商品检验检疫、制药公司、生物技术公司和食品工业等单位.齐一生物科技（上海）有限公司客服热线：021-60348496.Web:www.qiyibio.com
规 格：96T/48T
检测标本：血清,血浆,尿液,胸腹水,脑脊液,细胞培养上清,组织匀浆等
检测方法：ELISA
检测类型：酶联免疫夹心法
产品的用途：仅供科研究课题使用
价格及详细资料：电议,或咨询在线客服QQ741653262,或者以邮件形式发到我司邮箱qysw@qiyibio.com .齐一生物科技（上海）有限公司提供的ELISA试剂盒受到了广大科研单位的一致肯定和认同.大品牌保证,价格公道,倾力为国内外科研院校实验室提供质的产品.若有需要,我司将竭诚为您服务！本试剂盒用于测定血清,血浆及相关液体样本中含量.
实验原理：
本试剂盒应用双抗体夹心法测定标本中该产品水平.用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色.TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色.颜色的深浅和样品中的本产品呈正相关.用酶标仪在450nm波长下测定吸光度（OD值）,通过标准曲线计算样品中该产品浓度.
试剂盒组成：
试剂盒组成 48孔配置 96孔配置 保存
说明书 1份 1份
封板膜 2片（48） 2片（96）
密封袋 1个 1个
酶标包被板 1×48 1×96 2-8℃保存
标准品：1800ng/L 0.5ml×1瓶 0.5ml×1瓶 2-8℃保存
标准品稀释液 1.5ml×1瓶 1.5ml×1瓶 2-8℃保存
酶标试剂 3 ml×1瓶 6 ml×1瓶 2-8℃保存
样品稀释液 3 ml×1瓶 6 ml×1瓶 2-8℃保存
显色剂A液 3 ml×1瓶 6 ml×1瓶 2-8℃保存
显色剂B液 3 ml×1瓶 6 ml×1瓶 2-8℃保存
终止液 3ml×1瓶 6ml×1瓶 2-8℃保存
浓缩洗涤液 （20ml×20倍）×1瓶 （20ml×30倍）×1瓶 2-8℃保存
标本要求：
1．标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验.若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融
2．不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的（HRP）活性.
操作步骤：
1.标准品的稀释与加样：在酶标包被板上设标准品孔10孔,在第一、第二孔中分别加标准品100μl,然后在第一、第二孔中加标准品稀释液50μl,混匀；然后从第一孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀；然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀；混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉.（稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L）.
2.加样：分别设空白孔（空白对照孔不加样品及酶标试剂,其余各步操作相同）、待测样品孔.在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl（样品最终稀释度为5倍）.加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀.
3.温育：用封板膜封板后置37℃温育30分钟.
4.配液：将30（48T的20倍）倍浓缩洗涤液用蒸馏水30（48T的20倍）倍稀释后备用.
5.洗涤：小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干.
6.加酶：每孔加入酶标试剂50μl,空白孔除外.
7.温育：操作同3.
8.洗涤：操作同5.
9.显色：每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.
10.终止：每孔加终止液50μl,终止反应（此时蓝色立转黄色）.
11.测定：以空白空调零,450nm波长依序测量各孔的吸光度（OD值）. 测定应在加终止液后15分钟以内进行.
注意事项：
1．试剂盒从冷藏环境中取出应在室温平衡15-30分钟后方可使用,酶标包被板开封后如未用完,板条应装入密封袋中保存.
2．浓洗涤液可能会有结晶析出,稀释时可在水浴中加温助溶,洗涤时不影响结果.
3．各步加样均应使用加样器,并经常校对其准确性,以避免试验误差.一次加样时间控制在5分钟内,如标本数量多,推荐使用排抢加样.
4．请每次测定的同时做标准曲线,做复孔.如标本中待测物质含量过高（样本OD值大于标准品孔第一孔的OD值）,请先用样品稀释液稀释一定倍数（n倍）后再测定,计算时请最后乘以总稀释倍数（×n×5）.
5．封板膜只限一次性使用,以避免交叉污染.
6．底物请避光保存.
7．严格按照说明书的操作进行,试验结果判定必须以酶标仪读数为准.
8．所有样品,洗涤液和各种废弃物都应按传染物处理.
9．本试剂不同批号组分不得混用.
10. 如与英文说明书有异,以英文说明书为准.
计算：
以标准物的浓度为横坐标,OD值为纵坐标,
在坐标纸上绘出标准曲线,根据样品的OD
值由标准曲线查出相应的浓度；再乘以稀释
倍数；或用标准物的浓度与OD值计算出标
准曲线的直线回归方程式,将样品的OD值
代入方程式,计算出样品浓度,再乘以稀释
倍数,即为样品的实际浓度.
试剂盒性能：
1.样品线性回归与预期浓度相关系数R值为0.95以上.
2.批内与批见应分别小于9%和11%
保存条件及有效期：
1.试剂盒保存；2-8℃.
2．有效期：6个月
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Human Immunoreactive growth hormone,irGH ELISA试剂盒
Human melatonin sulfate,MS ELISA试剂盒
Human Long acting thyroid stimulator,LATS ELISA试剂盒
Human adrenomedullin,ADM ELISA试剂盒
Human Immunoreactive growth hormone,irGH ELISA试剂盒
Human antidiuretic hormone/vasopressin/arginine vasopressin,ADH/VP/AVP ELISA试剂盒
Human galanin,GAL ELISA试剂盒
Human secretin receptor,SR ELISA试剂盒
Human Estrogen Receptor,ER ELISA试剂盒
Human Ultra Sensitive growth hormone,U S-GH ELISA试剂盒
Human Ultra Sensitive Prostate Specific Antigen,PSA U S ELISA试剂盒
Human Nandrolone Phenylpropionate,NP ELISA试剂盒
Human 16-αHydroxyestrogen 1,16-α OHE-1 ELISA试剂盒
Human Hydrocortisone,HYD ELISA试剂盒
Human glucocorticoid receptor-β,GR-β ELISA试剂盒
Human glucocorticoid receptor-α,GR-α ELISA试剂盒
Human Ultrasensitivity Tri-iodothyronine,u-T3 ELISA试剂盒
Human Luteinizing Hormone-Releasing Hormone,LHRH ELISA试剂盒
Human 8-iso prostaglandin,8-iso-PG ELISA试剂盒
Human Renin ELISA试剂盒
Human Epinephrine/Adrenaline,EPI ELISA试剂盒
Human Estradiol receptor,ER ELISA试剂盒
Human Parathyroid Hormone Related Protein,PTHrP ELISA试剂盒
Human angiotensinogen,aGT ELISA试剂盒
Human angiotension Ⅰ,Ang-Ⅰ ELISA试剂盒
Human Follistatin,FS ELISA试剂盒
Human Parathyroid Hormone Related Protein,PTHrP ELISA试剂盒
Human catecholamine,CA ELISA试剂盒
Human Cystatin C,Cys-C ELISA试剂盒
Human acetylcholine receptor antibody,AChRab ELISA试剂盒
Human 5-Hydroxytryptamine,5HT ELISA试剂盒
Human Inhibin,INH-A ELISA试剂盒
Human sex hormone-binding globulin,SHBG ELISA试剂盒
Human Dehydroepiandrosterone S7,DHEA-S7 ELISA试剂盒
Human Dehydroepiandrosterone,DHEA ELISA试剂盒
Human calcitonin gene related peptide,CGRP ELISA试剂盒
Human Somatostatin,SS ELISA试剂盒
Human Growth Hormone Releasing Peptide,GHRP ELISA试剂盒
Human inhibin B,INH-B ELISA试剂盒
Human growth hormone releasing hormone,GHRH ELISA试剂盒
Human estrogen,E ELISA试剂盒
Human Melatonin,MT ELISA试剂盒
Human 6-keto-prostaglandin,6-K-PG ELISA试剂盒