库存 ：1000

供应商 ：康朗生物

检测限 ：见说明书

检测方法 ：酶联免疫

应用 ：科研

标记物 ：HRP标记物

样本 ：血清/组织/尿液

Human von Willebrand Factor cleaving protease,ADAMTS-13/vWF-cp ELISA Kit

标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected
5000 2.661 2.658 2.660 2.541
2500 2.164 2.122 2.143 2.024
1250 1.635 1.566 1.601 1.482
625 1.043 0.989 1.016 0.897
312 0.613 0.568 0.591 0.472
156 0.391 0.361 0.376 0.257
78 0.251 0.234 0.243 0.124
0 0.133 0.127 0.119

Human von Willebrand Factor cleaving protease,ADAMTS-13/vWF-cp ELISA Kit

精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Human von Willebrand Factor cleaving protease,ADAMTS-13/vWF-cp ELISA Kit

线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTS-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1000 Average % 88
Range % 80-92
1:2000 Average % 98
Range % 91-105
1:4000 Average % 101
Range % 92-110
1:8000 Average % 94
Range % 86-98

Human von Willebrand Factor cleaving protease,ADAMTS-13/vWF-cp ELISA Kit

回收率:
The recovery of human ADAMTS-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 89-98
EDTA plasma (n=4) 96 90-100