Cyclin D1 Antibody(CY5404)

免疫原 ：P24385

形态 ：34kDa

保存条件 ：-20度

适应物种 ：Human Mouse Rat

应用范围 ：WB IHC ICC/IF IP

抗体英文名 ：Cyclin D1 Antibody

规格 ：50ul/100ul

Product Datasheet		www.abways.com
Cyclin D1 Antibody Catalog No: CY5404 Reactivity: Human Mouse Rat Isotype: Rabbit IgG Applications: WB IHC ICC/IF IP
UniProt ID: P24385 All Names: CCND1;BCL1; D11S287E; PRAD1; U21B31; Cyclin D1;
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 34 kDa
WB: 1:1000~1:5000 IHC: 1:50~1:200 ICC/IF: 1:50~1:100
Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
Western blot analysis of Cyclin D1 expression in (1)MCF-7 cell lysates;(2) LnCaP cell lysates.
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