DYKDDDDK Tag Antibody标签抗体

英文名 ：DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368

库存 ：3

供应商 ：上海恒斐生物科技有限公司

CAS号 ：现货

规格 ：40ul

DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 说明书

Applications	Dilution	Species-Reactivity	Sensitivity	MW (kDa)	Isotype
W	1:1000		Transfected Only		Rabbit
IP	1:50
F	1:200
IF-IC	1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry)

Specificity / Sensitivity

DYKDDDDK Tag Antibody detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® antibodies) fused to either the amino- or carboxy-terminus of targeted proteins. 
 The binding specificity of this antibody is NOT dependent on the presence of divalent metal cations.

DYKDDDDK标签抗体（生物素化） 可以检测细胞中外源性表达DYKDDDDK的蛋白。此抗体可以识别与目的蛋白氨基末端或羧基末端的融合的DYKDDDDK肽段（与Sigma公司的抗FLAG抗体识别相同的表位）。此种抗体结合的特异性不依赖于二价金属离子的存在。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体是采用合成的DYKDDDDK肽段免疫动物而生产的(KLH-coupled) 。抗体采用蛋白A和亲和色谱法纯化。

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.

在免疫印迹、免疫沉淀和免疫染色等技术中，表位标签用于标记和检测蛋白。由于它们的体积很小，它们一般不会影响标记蛋白的生化特性。

The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1).

DYKDDDDK肽段作为常规的表位标签被广泛应用于表达载体中，此肽段可以通过与目的蛋白的氨基末端或羧基末端融合而被表达和检测（1）。

1. Brizzard, B. L. et al. (1994) Biotechniques 16, 730-735.

Application References

• Pfeffer, L.A. et al. (2009) Mol Biol Cell 20, 3810-7.Applications:IF-IC (In Cells)
• Forrester, M.T. et al. (2011) Biotechniques 50, 41-5.Applications:IF-IC (In Cells)
• Zhao, J.X. et al. (2011) J Biol Chem 286, 16426-34.Applications:Western Blotting
• Liu, C. et al. (2013) J Clin Invest 123, 1138-56.Applications:Western Blotting
• Menon, S. et al. (2014) Cell 156, 771-85.Applications:IF-IC (In Cells)