Magna ChIRP RNA Interactome Kit -Isolation and characterization of non-coding RNA:chromatin complexes价格

英文名：Magna ChIRP

供应商：上海恒斐生物科技有限公司

规格：12 Assays

Description
Catalogue Number	17-10494
Trade Name	Magna ChIRP
Description	Magna ChIRP RNA Interactome Kit -Isolation and characterization of non-coding RNA:chromatin complexes
Overview	Features and Advantages Recover non-coding RNA containing complexes using lncRNA and other types of chromatin associated RNA as targets Identify sites of genomic interaction of chromatin associated RNA Perform proteomic analysis of chromatin associated RNA regulatory complexes Includes PureProteome™ Streptavidin magnetic beads, plus all key buffers, enzymes, and reagents All kits include a negative control probe set and a detailed protocol EZ-Magna ChIRP kit includes a biotinylated TERC lncRNA positive control probe set and qPCR and RT-qPCR detection primer sets. Biotinylated capture probe sets for lncRNA TERC and NEAT1 sets are also available separately The Magna ChIRP RNA Interactome Kit uses the ChIRP method (Chromatin Isolation by RNA Purification) to enable identification and analysis of regions of chromatin that interact with a particular chromatin-associated RNA. This novel anti-sense probe-based capture strategy uses cross-linked chromatin to allow unbiased discovery of RNA-associated DNA sequences and proteins. To perform ChIRP two pools consisting of mulitple biotinylated oligonucleotide probes complementary to the RNA of interest are used (see figure 1). These probe sets are combined with chromatin and hybridized to the chromatin-associated RNA. Complexes containing biotinylated-probes bound to the chromatin-associated RNA are then isolated using streptavidin magnetic beads. DNA or RNA can then be recovered and analyzed by quantitative PCR or next generation sequencing (ChIRP-seq). Alternatively, proteomic analysis of the recovered chromatin can be performed. Learn more about the ChIRP method in JOVE! (Watch Now!) Need help making your own ChIRP probe sets? (Use the ChIRP probe designer tool.)

Product Information
Components	10X Glycine Magna ChIRP™ Negative Control Probe Set (LacZ) RNase H (10U/ µL) RNase A (20 mg/mL) DNase I Reaction Buffer DNase I RNase free Proteinase K Solution Protease Inhibitor Cocktail III RNase Inhibitor PureProteome™ Streptavidin magnetic beads 0.5M EDTA DNA Elution Buffer Proteinase K Buffer (for RNA) Proteinase K Buffer (for DNA) Wash Buffer Hybridization Buffer (without 15% formamide) Lysis Buffer
Presentation	Two boxes containing key reagents for extraction of ChIRP ready chromatin, plus magnetic beads enzymes, buffers,and biotinlyated negative control probes to allow the performance of 12 chromatin isolation by RNA purification assays.

Product Information

Components

10X Glycine
Magna ChIRP™ Negative Control Probe Set (LacZ)
RNase H (10U/ µL)
RNase A (20 mg/mL)
DNase I Reaction Buffer
DNase I RNase free
Proteinase K Solution
Protease Inhibitor Cocktail III
RNase Inhibitor
PureProteome™ Streptavidin magnetic beads
0.5M EDTA
DNA Elution Buffer
Proteinase K Buffer (for RNA)
Proteinase K Buffer (for DNA)
Wash Buffer
Hybridization Buffer (without 15% formamide)
Lysis Buffer

Presentation

Two boxes containing key reagents for extraction of ChIRP ready chromatin, plus magnetic beads enzymes, buffers,and biotinlyated negative control probes to allow the performance of 12 chromatin isolation by RNA purification assays.

Applications
Application	The Magna ChIRP RNA Interactome Kit allows analysis, and mapping of regions of chromatin interacting with chromatin-associated RNAs. Based on the ChIRP method (chromatin isolation by RNA purification), this probe-based capture assay enables discovery of RNA-associated DNA sequences and proteins.
Key Applications	DNA-binding Proteins Purification with magnetic beads Protein Interaction Assays Protein Determination DNA Sequencing RNA Detection

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed.