反转录试剂盒RevertAid First Strand cDNA Synthesis Kit

保存条件 ：42° C

英文名 ：RevertAid First Strand cDNA Synthesis Kit

供应商 ：上海恒斐生物科技有限公司

CAS号 ：现货促销中

规格 ：100 x 20 µL rxns

RevertAid First Strand cDNA Synthesis Kit 货号：K1622 说明书
synthesis of first strand cDNA from RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), a recombinant M-MuLV RT which maintains activity at 42-50°C and is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation.
The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of the poly(A) tail, therefore, they can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis of RNA species lacking a poly(A) tail (e.g., microRNAs). Gene-specific primers may also be used with the kit.

Reverse Transcriptase:	RevertAid Reverse Transcriptase
Downstream Application:	Reverse Transcriptase PCR (RT-PCR), Real-Time Quantitative PCR (qPCR)
Reaction Format:	Separate Components
Sample Type (General):	RNA
Product Size:	100 reactions
Optimal Reaction Temperature:	42° C