大鼠少突胶质前体细胞

The precursor cells for oligodendrocytes were first discovered in 1993 by Raff, Miller and Noble
[1] and have been extensively studied. These precursor cells are referred in the literature as either
oligodendrocyte-type-2 astrocyte progenitor cells or oligodendrocyte precursor cells (OPC). The
developing and adult central nervous system both contain OPC [2, 3]. Oligodendrocytes, the
myelin-forming cells of the central nervous system, develop from OPC. In culture, OPC can be
generated from neural progenitors or neural stem cells in the presence of basic fibroblast growth
factor and they proliferate in presence to platelet-derived growth factor or factors produced by
astrocytes [4] and differentiate into mature oligodendrocytes. Because of this, they have provided
an exceptional population in which to study developmental transitions.

ROPC from ScienCell Research Laboratories are isolated from neonate day 2 rat brain tissues. ROPC
are cryopreserved after purification and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml
volume. ROPC are characterized by immunofluorescent method with antibodies to A2B5 and nestin.
ROPC are negative for mycoplasma, bacteria, yeast and fungi. ROPC are guaranteed to further
culture at the conditions provided by ScienCell Research Laboratories.

It is recommended to use Oligodendrocyte Precursor Cell Medium (OPCM, Cat. #1601) for culturing ROPC in vitro and Oligodendrocyte Precursor Cell Differentiation Medium (OPCDM, Cat. #1631) for differentiating ROPC.

Product Use
ROPC are for research use only. It is not approved for human or animal use, or for application in in
vitro diagnostic procedures.

Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the
cells in liquid nitrogen until cell culture needed for experiments.

Shipping
Dry ice.

Reference

[1] Raff, M. C., Miller, R. H. and Noble, M. (1983) A glial progenitor cell that develops in vitro into an
astrocyte or an oligodendrocyte depending on the culture medium. Nature 303:390-396.
[2] ffrench-Constant, C. and Raff. M. C. (1986) Proliferating bipotential glial progenitor cells in adult
rat optic nerve. Nature 319:499-502.
[3] Wolswijk, G. and Noble, M. (1989) Identification of an adult-specific glial progenitor cell.
Development 105:387-400.
[4] Noble, M., Murray, K., Stroobant, P., Waterfield, M. D. Riddle, P. (1988) Platelet-derived growth
factor promotes division and motility and inhibits premature differentiation of the oligodendrocyte
/type-2 astrocyte progenitor cells. Nature 333:560-562