人小脑星形胶质细胞

Astrocytes make up the majority of the cells in the mammalian brain. They are the most variable in type,
most intimately associated with all parts of neurons, and thus most functionally interesting in their
relationships with neurons [1]. They provide structural, trophic, and metabolic support to neurons and
modulate synaptic activity. Impairment of these astrocyte functions during stroke and other insults can
critically influence neuron survival. Furthermore, astrocytes have been implicated in the pathological processes
of many neurological diseases [2]. Long-term recovery after brain injury, through neurite outgrowth, synaptic
plasticity, or neuron regeneration, is influenced by astrocyte surface molecule expression and trophic factor
release [3]. In addition, the death or survival of astrocytes themselves may affect the ultimate clinical outcome.
Recognition of the importance of astrocytes in nervous system functioning is increasing, specifically regarding
the modulation of neural activity. Much of what we have learned about astrocytes is from the in vitro studies
and astrocyte cultures are continuing to provide a useful tool in exploring the diverse property of these cells.

HA-c from ScienCell Research Laboratories are isolated from human cerebellar. HA-c are cryopreserved at
passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HA-c are characterized
by immunofluorescent method with antibody to GFAP. HA-c are negative for HIV-1, HBV, HCV, mycoplasma,
bacteria, yeast and fungi. HA-c are guaranteed to further expand for 15 population doublings at the
conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Astrocyte Medium (AM, Cat. No. 1801) for the culturing of HA-c in vitro.

Product Use

HA-c are for research use only. It is not approved for human or animal use, or for application in in vitro
diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in
liquid nitrogen until cell culture is needed for experiments.

Shipping

Dry ice.

Reference

[1] G. I. Hatton (2002) Glial-neuronal interactions in the mammalian brain. Adv. in Physiol. Edu. 26:225-237.
[2] Van der Laan, L. J. W., De Groot, C. J. A., Elices, M. J. and Dijkstran, C. D. (1997) Extracellular matrix
proteins expressed by human adult astrocytes in vivo and in vitro: an astrocyte surface protein containing
the CS1 domain contributes to binding of lymphoblasts. J. Neurosci. Res. 50:539-548.
[3] Chen Y., and Swanson, R. A. (2003) Astrocytes and brain injury. J. Cereb. Blood Flow Metab. 23:137-149.