人脑膜细胞

HMC from ScienCell Research Laboratories are isolated from human leptomeningi. HMC are cryopreserved
at primary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HMC are
characterized by immunofluorescent method with antibodies to fibronectin and negative to GFAP, α-smooth
muscle actin and Thy 1.1. HMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
HMC are guaranteed to further expand for 5 population doublings at the condition provided by ScienCell
Research Laboratories.

Recommended Medium

It is recommended to use Meningeal Cell Medium (MCM, Cat. No. 1401) for the culturing of HMC in vitro.

Product Use

HMC are for research use only. It is not approved for human or animal use, or for application in in vitro
diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in
liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1] Hartmann, D., Sievers, J. Pehlemann, F. W. and Berry, M. (1992) Destruction of meningeal cells over the
medial cerebral hemisphere of newborn hamster prevents the formation of the infrapyramidal blade of the
dentate gyrus. J. Comparative Neurol. 320:33-61.
[2] Franzen, R., Martin, D., Daloze, A., Moonen, G. and Schoenen, J. (1999) Grafts of meningeal fibroblasts
in adult rat spinal cord lesion promote axonal regrowth. Neuroreport 10:1551-1556.
[3] Hartmann, D., Schulze, M. and Sievers, J. (1998) Meningeal cells stimulate and direct the migration of
cerebellar external granule cells in vitro. J. Neurocytol. 27:395-409.