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形态 ： 液态/粉状
保存条件 ： -20℃
克隆性 ：多克隆
适应物种 ： 人，大鼠，小鼠，兔.......
宿主 ： Goat，Rabbit，Mouse
应用范围 ： 科研使用
浓度 ： 1mg/ml
靶点 ： 来电咨询
抗体英文名 ：Polyclonal Antibody to Fibronectin (FN)
抗体名 ：纤连蛋白(FN)多克隆抗体
适用生物 Homo sapiens(人，小鼠，大鼠)纤连蛋白(FN)多克隆抗体
产品应用 WB, ICC, IHC-P, IHC-F, ELISA 规格 100ug
标记物 未标记 价格 请咨询当地代理商
浓度 200ug/ml
克隆类型 多克隆 宿主 Rabbit，兔
纤连蛋白(FN)多克隆抗体
Polyclonal Antibody to Fibronectin (FN)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | PAA037Hu01 |
| Clonality | Polyclonal |
| Host | Rabbit |
| Immunoglobulin Type | IgG |
| Purification | Affinity Chromatography |
| Applications | WB, ICC, IHC-P, IHC-F, ELISA |
| Concentration | 200ug/ml |
| UOM | 100ug |
| Conjugate | No Conjugate |
IMMUNOGEN INFORMATION
纤连蛋白(FN)多克隆抗体Immunogen: Recombinant fibronectin (Gly313~Ser607) expressed in E.coli.
Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- GVVYSVGM QWLKTQGNKQ MLCTCLGNGV SCQETAVTQT YGGNSNGEPC VLPFTYNGRT FYSCTTEGRQ DGHLWCSTTS NYEQDQKYSF CTDHTVLVQT RGGNSNGALC HFPFLYNNHN YTDCTSEGRR DNMKWCGTTQ NYDADQKFGF CPMAAHEEIC TTNEGVMYRI GDQWDKQHDM GHMMRCTCVG NGRGEWTCIA YSQLRDQCIV DDITYNVNDT FHKRHEEGHM LNCTCFGQGR GRWKCDPVDQ CQDSETGTFY QIGDSWEKYV HGVRYQCYCY GRGIGEWHCQ PLQTYPS
Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- GVVYSVGM QWLKTQGNKQ MLCTCLGNGV SCQETAVTQT YGGNSNGEPC VLPFTYNGRT FYSCTTEGRQ DGHLWCSTTS NYEQDQKYSF CTDHTVLVQT RGGNSNGALC HFPFLYNNHN YTDCTSEGRR DNMKWCGTTQ NYDADQKFGF CPMAAHEEIC TTNEGVMYRI GDQWDKQHDM GHMMRCTCVG NGRGEWTCIA YSQLRDQCIV DDITYNVNDT FHKRHEEGHM LNCTCFGQGR GRWKCDPVDQ CQDSETGTFY QIGDSWEKYV HGVRYQCYCY GRGIGEWHCQ PLQTYPS
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against FN. It has been selected for its ability to recognize FN in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4,
containing 0.02% NaN3, 50% glycerol.
containing 0.02% NaN3, 50% glycerol.
IHC Images
QUALITY CONTROL
纤连蛋白(FN)多克隆抗体Content: The quality control contains recombinant fibronectin (Gly313~Ser607) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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