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库存 ： 1000
供应商 ： 上海钰博
检测限 ： 见说明书
检测方法 ： 酶联免疫
应用 ： 科研
标记物 ： 见说明书
样本 ： 血清/组织/尿液
适用生物 Homo sapiens (Human，人)转化生长因子β1(TGFβ1)检测试剂盒
检测范围 31.25-2000pg/mL 灵敏度 11.5pg/mL
样本类型 Serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
转化生长因子β1(TGFβ1)检测试剂盒
ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | SEA124Hu |
| Sample type | Serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 31.25-2000pg/mL The standard curve concentrations used for the ELISA’s were 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 11.5pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 1 (TGFb1).
No significant cross-reactivity or interference between Transforming Growth Factor Beta 1 (TGFb1) and analogues was observed.
No significant cross-reactivity or interference between Transforming Growth Factor Beta 1 (TGFb1) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Transforming Growth Factor Beta 1 (TGFb1) and the recovery rates were calculated by comparing the measured value to the expected amount of Transforming Growth Factor Beta 1 (TGFb1) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 83-101 | 90 |
| EDTA plasma(n=5) | 92-99 | 96 |
| heparin plasma(n=5) | 81-103 | 101 |
Precision
转化生长因子β1(TGFβ1)检测试剂盒Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Transforming Growth Factor Beta 1 (TGFb1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 98-105% | 97-105% | 92-101% |
| EDTA plasma(n=5) | 99-105% | 96-104% | 85-105% | 98-105% |
| heparin plasma(n=5) | 87-96% | 83-96% | 86-101% | 83-95% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
转化生长因子β1(TGFβ1)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta 1 (TGFb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transforming Growth Factor Beta 1 (TGFb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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