Stemolecule™ Doxycycline hyclate

保存条件 ： 4℃保存

保质期 ： 6个月

英文名 ：Stemolecule™ Doxycycline hyclate

库存 ： 1

供应商 ： 上海信裕

Stemolecule™ Doxycycline hyclate详细信息：
Product Overview

Stemolecule™ Doxycycline hyclate (Dox) is a broad spectrum antibiotic derivative of tetracycline and an inhibitor of matrix metalloproteinases in vivo. Tetracycline-controlled transcriptional activation is a method of inducible expression whereby transcription is reversibly turned on or off in the presence of tetracycline or one of its derivatives such as Dox1. Dox-inducible lentiviral reagents such as the Stemgent® iPSC Generation Dox Inducible Lentivirus products are used to induce the expression of virally transduced genes and generate induced pluripotent stem (iPS) cells from somatic cells (in a process referred to as reprogramming2,3) by adding Dox to the cell culture medium4-9. Stemolecule™ Doxycycline hyclate is the recommended inducer for all of the Stemgent® iPSC Generation Dox Inducible products. In the iPSC generation protocol, addition of Dox to the culture medium of transduced cells induces the reverse tetracycline transactivator (rtTA) to bind to the Tet response element and results in the transcriptional activation for each of the factors implicated in the reprogramming process. Conversely, Dox can be removed from the medium to suppress transcriptional activation of the reprogramming factors.

Stemolecule™ Doxycycline hyclateProduct Specifications

Size 10 mg
Lot Number 1203
Quantity / Size 10 mg
Alternate Names (4S,4aR,5S,5aR,6R,12aS)-4-(dimethylamino)-3,5,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-1,4,4a,5,5a,6,11,12a-octahydrotetracene-2-carboxamide; Doxycycline hydrochloride hemiethanolate hemihydrate; Dox
Chemical Formula C22H24N2O8 • HCl • ½(H2O) • ½(C2H6O)
Structure
Stemolecule™ Doxycycline hyclateMolecular Weight 512.94
CAS Number 24390-14-5
Purity ≥98% (TLC)
Formulation Yellow to yellow with a green cast powder
Solubility Soluble in water
*DMSO upper limit tolerance is <0.5% in most cells.
Storage Store at 4°C. Protect from light. Following reconstitution, store aliquots at -20°C.
Stability Stock solutions stable at -20°C for 6 months.
Quality control The purity of Stemolecule™ Doxycycline hyclate was determined by TLC analysis. The accurate mass and structure were determined by mass spectrometry and NMR, respectively. Cellular toxicity was tested on mouse embryonic stem cells.
Stemolecule™ Doxycycline hyclate
xy10278 小鼠T淋巴细胞瘤细胞,Cyc-Tag(S49)细胞
xy10279 小鼠T淋巴细胞,Cl.Ly1+2-/9细胞
xy10280 小鼠SRSV转化的3T3细胞,SRSV/3T3细胞
xy10281 小鼠Mo-MuLv感染的3T3细胞,Mo-MuLV/3T3细胞
xy10282 小鼠Lewis肺癌细胞,LLC-RFP-puro细胞
xy10283 小鼠Lewis肺癌瘤株 LLT细胞
xy10284 小鼠L6565白血病克隆细胞系 L6565细胞
xy10285 小鼠IL-3依赖性32D细胞
xy10286 小鼠B细胞杂交瘤细胞,W6/32细胞
xy10287 小鼠B淋巴细胞杂交瘤细胞,SH3细胞
xy10288 小鼠B淋巴细胞,WEHI 231细胞
xy10289 小儿包皮细胞,HFF细胞
xy10290 小肠结肠炎耶尔森氏菌
xy10291 涎腺腺样囊性癌细胞,Acc-2细胞
xy10292 鲜绿青霉
xy10293 细胞简称
xy10294 细胞毒性T淋巴细胞,CTLL-2细胞,
xy10295 吸水链霉菌应城变种
xy10296 吸水链霉菌奥萨霉素亚种
xy10297 稳定转染了neor和LIF基因的STO细胞株 SNL细胞
xy10298 稳定表达EBNA1的人胚肾细胞,293E细胞
xy10299 蚊子细胞,C6/36细胞
xy10300 胃腺癌细胞（低分化）
xy10301 胃腺癌细胞,SGC-7901细胞
xy10302 胃腺癌细胞,AGS细胞
xy10303 胃癌细胞,MKN-74细胞
xy10304 胃癌细胞,MKN28细胞
xy10305 胃癌细胞,MGC80-3细胞
xy10306 胃癌细胞,MFC细胞
xy10307 宛氏拟青霉
xy10308 脱氮假单胞菌
xy10309 脱氮付球菌
xy10310 团头鲂尾鳍细胞,WCF细胞
xy10311 兔子垂体细胞
xy10312 兔主动脉平滑肌细胞,SMC细胞
xy10313 兔主动脉平滑肌细胞,CCC-SMC-2细胞
xy10314 兔眼Tenon＇s囊成纤维细胞,RYTF细胞
xy10315 兔肾细胞系 RK-13细胞
xy10316 兔晶体上皮细胞永生系 N/N1003A（RLE）细胞
xy10317 兔角膜前基质层成纤维细胞,RCFBF细胞
xy10318 兔肝癌细胞,VX2细胞,
xy10319 兔成骨C IF细胞
xy10320 土星汉逊酵母
xy10321 土壤伯克氏菌