兔抗人β-2微球蛋白的多克隆抗体

保存条件 ：-20温度

英文名 ：Rabbit anti-human beta-2-microglobulin polyclonal Antibody

库存 ：大量现货

供应商 ：上海笃玛生物科技有限公司

规格 ：50μl/150μl

上海笃玛生物免疫组化实验方法说明书

兔抗人β-2微球蛋白的多克隆抗体免疫组化方法的具体步骤为：
1、取材：取实验组和对照组动物组织尽可能新鲜，PBS(磷酸盐缓冲液)洗，取小于0.5cm×0.5cm×0.1cm组织块，
2、固定和包埋：用4%多聚甲醛（0.1MPBS,PH7.0～7.6,含0.1%DEPC）进行固定，经70%乙醇30分钟、80%乙醇30分钟、90%乙醇30分钟两次、95%乙醇30分钟两次、100%乙醇30分钟两次、二甲苯透明30分钟两次、55℃石蜡中30分钟两次、用铜制模具包埋组织块；
3、切片：将厚度5um的组织切片附于经多聚赖氨酸附膜的载玻片上，60℃过夜；
4、脱蜡、入水：将切片浸于二甲苯中5分钟两次、100%乙醇5分钟两次、95%乙醇5分钟两次、90%乙醇5分钟两次、85%乙醇5分钟两次、75%乙醇5分钟两次、自来水冲洗、PBS洗两次；
5、1%甲醇双氧水,室温10分钟, 蒸馏水洗1次,0.1MPBS洗3次×5分钟;
6、切片上滴加抗原修复液,室温10分钟，0.1MPBS洗3次×5分钟;
7、切片上滴加正常山羊血清封闭液，室温20分钟。甩去多余液体，不洗。
8、切片上滴加第一抗体，4℃过夜，0.1MPBS洗3次×5分钟。
9、切片上滴加生物素化第二抗体（IgG），37℃20分钟，0.1MPBS洗3次×5分钟；
10、切片上滴加辣根酶标记链霉卵白素工作液（S-A/HRP），37℃20分钟，0.1MPBS洗3次×5分钟；
11、DAB显色：使用DAB显色试剂盒1ml蒸馏水加显色剂A、B、C各一滴，混匀，加至标本上，显色6分钟，充分水洗；
12、苏木素复染细胞核1分钟，充分水洗、1%盐酸酒精分化、1%胺水反蓝、充分水洗、经70%乙醇5分钟、80%乙醇5分钟、90%乙醇5分钟两次、95%乙醇5分钟两次、100%乙醇5分钟两次脱水、二甲苯透明5分钟两次、中性树脂封片，
13、显微镜观察：选择实验组和对照组的阳性和阴性组织相、进行100×和400×的显微照相。14、图象分析：选择有意义的组织相，经登录、编号、采集、分析、读取数据、最后存盘。

还有以下优惠产品：

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兔抗人ATP酶，H+的输送，溶酶体38kDa，V0亚基D1多克​​隆抗体

Rabbit anti-human ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A polyclonal Antibody
兔抗人ATP酶，H+运输，溶酶体的70kDa，V1亚基的多克隆抗体

Rabbit anti-human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1 polyclonal Antibody
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Rabbit anti-human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 polyclonal Antibody
兔抗人ATP酶，H+的输送，溶酶体56/58kDa，V1亚基B2多克隆抗体

Rabbit anti-human ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1 polyclonal Antibody
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Rabbit anti-human ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C2 polyclonal Antibody
兔抗人ATP酶，H+的输送，溶酶体42kDa，V1亚基C2多克隆抗体

Rabbit anti-human ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D polyclonal Antibody
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Rabbit anti-human ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1 polyclonal Antibody
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Rabbit anti-human ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2 polyclonal Antibody
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Rabbit anti-human ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F polyclonal Antibody
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Rabbit anti-human ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1 polyclonal Antibody
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Rabbit anti-human ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G3 polyclonal Antibody
兔抗人ATP酶，H+的输送，溶酶体13kDa，V1亚基G3多克隆抗体

Rabbit anti-human ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1 polyclonal Antibody
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Rabbit anti-human ATPase, class II, type 9A polyclonal Antibody
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Rabbit anti-human ATP synthase mitochondrial F1 complex assembly factor 1 polyclonal Antibody
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Rabbit anti-human ATP synthase mitochondrial F1 complex assembly factor 2 polyclonal Antibody
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Rabbit anti-human arginine vasopressin-induced 1 polyclonal Antibody
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Rabbit anti-human antizyme inhibitor 1 polyclonal Antibody
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Rabbit anti-human beta-2-microglobulin polyclonal Antibody
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Rabbit anti-human beta-1,3-N-acetylgalactosaminyltransferase 2 polyclonal Antibody
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Rabbit anti-human UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 4 polyclonal Antibody
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Rabbit anti-human UDP-Gal:betaGal beta 1,3-galactosyltransferase polypeptide 6 polyclonal Antibody
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Rabbit anti-human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3 polyclonal Antibody
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Rabbit anti-human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 2 polyclonal Antibody
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Rabbit anti-human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3 polyclonal Antibody
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Rabbit anti-human UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4 polyclonal Antibody
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Rabbit anti-human bile acid Coenzyme A: amino acid N-acyltransferase (glycine N-choloyltransferase) polyclonal Antibody
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Rabbit anti-human beta-site APP-cleaving enzyme 1 polyclonal Antibody
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Rabbit anti-human beta-site APP-cleaving enzyme 2 polyclonal Antibody
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Rabbit anti-human BCL2-associated agonist of cell death polyclonal Antibody
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Rabbit anti-human BCL2-associated athanogene polyclonal Antibody
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Rabbit anti-human BCL2-associated athanogene 3 polyclonal Antibody
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Rabbit anti-human BCL2-associated athanogene 4 polyclonal Antibody
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Rabbit anti-human BMP and activin membrane-bound inhibitor homolog (Xenopus laevis) polyclonal Antibody
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Rabbit anti-human BTG3 associated nuclear protein polyclonal Antibody
兔抗人BTG3相关核蛋白多克隆抗体

Rabbit anti-human BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) polyclonal Antibody
兔抗人BRCA1相关蛋白-1（泛素羧基末端水解酶）的多克隆抗体

Rabbit anti-human basic leucine zipper transcription factor, ATF-like polyclonal Antibody
兔抗人碱性亮氨酸拉链转录因子，ATF状多克隆抗体

Rabbit anti-human Apoptosis regulator BAX(BAX) polyclonal Antibody
兔抗人凋亡调节BAX（BAX）多克隆抗体