氨基酸和肉碱检测试剂盒（非衍生化 串联质谱法LC-MS/MS ）

供应商 ：祥生兴业

规格 ：960 analyses

新生儿遗传代谢疾病检测，串联质谱分析方法试剂盒LC-MS/MS 非衍生化

• All required and isotope labelled standards included

• Dried blood spot controls included

• Direct sample injection without derivatisation

• High selectivity and sensitivity by MS/MS detection

This Chromsystems reagent kit allows the very fast and reliable determination of amino acids and acylcarnitines from dried blood spot samples as part of the newborn screening for amino acid and fatty acid metabolic disorders using tandem mass spectrometry. Sample preparation is based on effective extraction of the analytes from the filter card without any further sample preparation steps required. The test utilises stable isotope labelled internal standards for calibration and measurement to ensure reliable and precise quantification of the analytes. Due to the time saving sample preparation and high precision of the MRM method, this direct injection kit stands out from the derivatised method. On the other hand lower sensitivity and interferences from isobaric substances (e.g. C5DC and C6OH) in acylcarnitines analyses may occur.

Specifications

Linearity:	Amino acids: 2000-2400 µmol/l (Acyl-)carnitines: 25-200 µmol/l
Limit of quantification:	Amino acids: 2.5-30 µmol/l (Acyl-)carnitines: 0.1-1.6 µmol/l
Recovery:	Amino acids: 64-94 % (Acyl-)carnitines: 63-102 %
Intra-assay:	Amino acids: 2-11 % (Acyl-)carnitines: 3-12 %
Inter-assay:	Amino acids: 3.5-18 % (Acyl-)carnitines: 4.8-14 %
Analysis time:	< 2 min

Cutoff thresholds

Amino acids:	ca. 30 to 1000 µmol/l
free carnitine:	ca. 55 µmol/l
Acylcarnitines:	ca. 0.2 to 10 µmol/l

LC-MS/MS parameters

LC-MS/MS system. No HPLC column is required.

Injection volume:	10 µl
Flow gradient:	20 to 600 µl/min
Autosampler rinsing solution:	Rinsing Solution (57007)
MS/MS Mode:	MRM
Mass transitions:
Alanine	90 > 44
Alanine-D4	94 > 48
Arginine	175 > 70
Arginine-D7	182 > 77
Aspartic acid	134 > 116
Aspartic acid-D3	137 > 119
Citrulline	176 > 113
Citrulline-D2	178 > 115
Glutamic acid	148 > 130
Glutamic acid-D5	153 > 135
Glycine	76 > 30
Glycine-13C2-15N	79 > 32
Leucine	132 > 86
Leucine-D3	135 > 89
Methionine	150 > 133
Methionine-D3	153 > 136
Ornithine	133 > 70
Ornithine-D6	139 > 76
Phenylalanine	166 > 120
Phenylanlanine-D5	171 > 125
Proline	116 > 70
Proline-D7	123 > 77
Tyrosine	182 > 136
Tyrosine-D4	186 > 140
Valine	118 > 72
Valine-D8	126 > 80
Free carnitine	162 >85
Carnitine-D9	171 > 85
C2-Carnitine	204 > 85
C2-Carnitine-D3	207 > 85
C3-Carnitine	218 > 85
C3-Carnitine-D3	221 > 85
C4-Carnitine	232 > 85
C4-Carnitine-D3	235 > 85
C5-Carnitine	246 > 85
C5-Carnitine-D9	255 > 85
C5DC-Carnitine	276 > 85
C5DC-Carnitine-D6	282 > 85
C6-Carnitine	260 > 85
C6-Carnitine-D3	263 > 85
C8-Carnitine	288 > 85
C8-Carnitine-D3	291 > 85
C10-Carnitine	316 > 85
C10-Carnitine-D3	319 > 85
C12-Carnitine	344 > 85
C12-Carnitine-D3	347 > 85
C14-Carnitine	372 > 85
C14-Carnitine-D3	375 > 85
C16-Carnitine	400 > 85
C16-Carnitine-D3	403 > 85
C18-Carnitine	428 > 85
C18-Carnitine-D3	431 > 85

Sample preparation

• Punch 3 mm dried blood spot disk out of the filter card into a well plate.
• Add 100 µl Internal Standard, agitate for 20 min.
• Inject 10 µl of the extract into the LC-MS/MS system.

Order no.	Product
57000	MassChrom® Reagent kit for the determination of amino acids and acylcarnitines from dried blood spot (LC-MS/MS)/Non Derivatised with regular 96 well plates. For 960 analyses.
57000/F	MassChrom® Reagent kit for the determination of amino acids and acylcarnitines from dried blood spot (LC-MS/MS)/Non Derivatised with 96 well filter plates. For 960 analyses.
	Separately available components
57001	Mobile Phase, 1000 ml
57002	Mobile Phase, 10 x 1000 ml
57004/T	Internal Standard (lyoph.), 25 ml
57004	Internal Standard (lyoph.), 4 x 25 ml
57007	Rinsing Solution, 1000 ml
57008	Extraction Buffer, 100 ml
57010	96-Well-Plates, V-Boden, 5 pcs.
55011	Protective sheets for 96 well plates, 10 pcs.
57057	96 Well filter plates, 5 pcs.
55013	Piercable adhesive seal for 96 well plates, 10 pcs.
	Startup accessories
55015	Restrictor Capillary, 1 pc.
55016	Adapter collar for centrifugation, 2 pcs.
57099	Tuning Mix, incl. all analytes, 2 ml
55033	PEEK prefilter, 5 µm, 5 pcs.
15010	PEEK prefilter housing, 1 pc.
	Chromsystems quality control material (filter spot)
0191	MassCheck® Amino Acids, Acylcarnitines Dried Blood Spot Control, Bi-Level (I+II), 2 x 3 Spots
0192	MassCheck® Amino Acids, Acylcarnitines Dried Blood Spot Control, Level I, 1 x 3 spots
0193	MassCheck® Amino Acids, Acylcarnitines Dried Blood Spot Control, Level II, 1 x 3 spots