乙酰化组蛋白H3免疫沉淀试剂盒

保存条件 ： 4℃保存

保质期 ： 6个月

英文名 ：EpiQuik Acetyl-Histone H3 ChIP Kit

库存 ： 1

供应商 ： 上海信裕

• 乙酰化组蛋白H3免疫沉淀试剂盒详细信息：
• Product Overview
  
  The EpiQuik™ Acetyl-Histone H3 ChIP Kit is a convenient package of tools that allows the experimenter to investigate interactions of histone H3 acetylation and DNA in vivo efficiently. The entire procedure can be completed within 5 hours and produces far superior results than any competitor kits. The EpiQuik™ Acetyl-Histone H3 ChIP kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot as well as DNA microarray.
  
  WHY CHOOSE THE EPIQUIK™ ACETYL-HISTONE H3 ChIP KIT?
  • The fastest procedure available, which can be finished within 5 hours.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included: save time and reduce labor.
  • Compatible with all DNA amplification-based approaches.
  • Simple, reliable, and consistent assay conditions.
  乙酰化组蛋白H3免疫沉淀试剂盒Principle & Procedure

  
  The EpiQuik™ Acetyl-Histone H3 ChIP kit contains all reagents required for carrying out a successful chromatin immunoprecipitation for acetyl-histone H3 from mammalian cells. Particularly, this kit includes a ChIP-grade acetyl-histone H3 antibody and a negative control normal mouse IgG. Chromatin in the cells is extracted, sheared and added into the microwell immobilized with the antibody. Acetyl-histone H3 protein-DNA complex is captured by the antibody and DNA is released, and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.
  

  SCHEMATIC PROCEDURE:

  乙酰化组蛋白H3免疫沉淀试剂盒Product Components

  
  CP1 (wash buffer)
  CP2 (antibody buffer)
  CP3A (pre-lysis buffer)
  CP3B (lysis buffer)
  CP4 (CHIP dilution buffer)
  CP5 (DNA release buffer)
  CP6 (reverse buffer)
  CP7 (binding buffer)
  CP8 (elution buffer)
  Protease inhibitor cocktail (100x)*
  Proteinase K (10 mg/ml)*
  Normal mouse IgG (0.5 mg/ml)*
  Anti-acetyl-histone H3 (1 mg/ml)*
  8-well assay strips (with frame)
  8-well strip caps
  F-spin column
  F-collection tube
  User guide
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  * Spin the solution down to the bottom before use.
  乙酰化组蛋白H3免疫沉淀试剂盒User Guide & MSDS