组织甲基组蛋白H3-K4免疫沉淀试剂盒

保存条件 ： 4℃保存

保质期 ： 6个月

英文名 ：EpiQuik Tissue Methyl-Histone H3-K4 ChIP Kit

库存 ： 1

供应商 ： 上海信裕

• 组织甲基组蛋白H3-K4免疫沉淀试剂盒详细信息：
• Product Overview
  
  EpiQuik™ Tissue Methyl-Histone H3-K4 ChIP Kit is a convenient package of tools that allows the experimenter to investigate interactions of histone H3-k4 methylation and DNA in variety of mammalian tissues. The entire procedure can be completed within 5 hours and produces far superior results than any competitor kits. The EpiQuik™ Tissue Methyl-Histone H3-K4 ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot as well as DNA microarray.
  
  WHY CHOOSE THE EPIQUIK™ TISSUE METHYL-HISTONE H3-K4 ChIP KIT?
  
  • The fastest procedure available, which can be finished within 5 hours.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included: save time and reduce labor.
  • Compatible with all DNA amplification-based approaches.
  • Simple, reliable, and consistent assay conditions.
  组织甲基组蛋白H3-K4免疫沉淀试剂盒Principle & Procedure

  
  The EpiQuik™ Tissue Methyl-Histone H3-K4 ChIP Kit contains all reagents required for carrying out a successful chromatin immunoprecipitation for methyl-histone H3-K4 from mammalian tissues. Particularly, this kit includes a ChIP-grade methyl-histone H3-K4 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured methyl-histone H3-K4 protein-DNA complex, reversed and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.
  

  SCHEMATIC PROCEDURE:

  组织甲基组蛋白H3-K4免疫沉淀试剂盒Product Components

  
  CP1 (wash buffer)
  CP2 (antibody buffer)
  CP3 (lysis buffer)
  CP4 (CHIP dilution buffer)
  CP5 (DNA release buffer)
  CP6 (reverse buffer)
  CP7 (binding buffer)
  CP8 (elution buffer)
  Homogenizing buffer
  Protease inhibitor cocktail (100x)*
  Proteinase K (10 mg/ml)*
  Normal mouse IgG (0.5 mg/ml)*
  Anti-dimethyl-H3-K4 (1 mg/ml)*
  8-well assay strips (with frame)
  8-well strip caps
  F-spin column
  F-collection tube
  User guide
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  xy-E11334 细胞毒素(CTX)ELISA试剂盒 检测范围
  xy-E11335 α突触核蛋白(α-SYN)ELISA试剂盒 检测范围
  xy-E11336 糖链抗原19-9(CA19-9)ELISA试剂盒 检测范围
  xy-E11337 水蛭素(HRD)ELISA试剂盒 检测范围
  xy-E11338 他克莫司(FK506)ELISA试剂盒 检测范围
  xy-E11339 上皮特异性抗原(ESA)ELISA试剂盒 检测范围
  xy-E11340 上皮膜抗原(EMA)ELISA试剂盒 检测范围
  xy-E11341 凝聚素(CLU)ELISA试剂盒 检测范围
  xy-E11342 OJ抗体/抗异亮氨酰tRNA合成酶抗体(OJ/IleRS)ELISA试剂盒 检测范围
  xy-E11343 抗着丝点抗体(ACA/CENP)ELISA试剂盒 检测范围
  xy-E11344 肌细胞生成素(MYOG)ELISA试剂盒 检测范围
  xy-E11345 A组链球菌菌壁多糖抗体(ASP)ELISA试剂盒 检测范围
  xy-E11346 活化蛋白C抵抗素(APCR)ELISA试剂盒 检测范围
  xy-E11347 N端外显肽(Ext-N)ELISA试剂盒 检测范围
  xy-E11348 分泌成分(SC)ELISA试剂盒 检测范围
  xy-E11349 二磷酸腺苷(ADP)ELISA试剂盒 检测范围
  xy-E11350 蛋白聚糖(PG)ELISA试剂盒 检测范围
  xy-E11351 吡啶酚(PYD)ELISA试剂盒 检测范围
  xy-E11352 5羟基吲哚乙酸(5-HIAA)ELISA试剂盒 检测范围
  xy-E11353 12羟二十烷四烯酸(12-HETE)ELISA试剂盒 检测范围
  xy-E11354 羟脯氨酸(Hyp)ELISA试剂盒 检测范围
  xy-E11355 一氧化氮(NO)ELISA试剂盒 检测范围
  xy-E11356 上皮中性粒细胞活化肽78(ENA-78/CXCL5)ELISA试剂盒 检测范围
  xy-E11357 维生素C(VC)ELISA试剂盒 检测范围
  xy-E11358 维生素B6(VB6)ELISA试剂盒 检测范围
  xy-E11359 可溶性P选择素(sP-selectin)ELISA试剂盒 检测范围
  xy-E11360 基质金属蛋白酶抑制因子1(TIMP-1)ELISA试剂盒 检测范围
  xy-E11361 维生素D(VD)ELISA试剂盒 检测范围
  xy-E11362 维生素K1(VK1)ELISA试剂盒 检测范围
  xy-E11363 维生素B12(VB12)ELISA试剂盒 检测范围
  xy-E11364 维生素D3(VD3)ELISA试剂盒 检测范围
  xy-E11365 维生素D2(VD2)ELISA试剂盒 检测范围
  xy-E11366 维生素E(VE)ELISA试剂盒 检测范围
  xy-E11367 维生素A(VA)ELISA试剂盒 检测范围
  xy-E11368 鸟苷酸交换因子(GEF)ELISA试剂盒 检测范围
  xy-E11369 维生素D受体(VD R)ELISA试剂盒 检测范围
  xy-E11370 毒性休克综合征毒素1(TSST-1)ELISA试剂盒 检测范围
  * Spin the solution down to the bottom before use.
  组织甲基组蛋白H3-K4免疫沉淀试剂盒User Guide & MSDS