Tri-Methyl-Histone H3-K9免疫沉淀试剂盒

保存条件 ： 4℃保存

保质期 ： 6个月

英文名 ：EpiQuik Tri-Methyl-Histone H3-K9 ChIP Kit

库存 ： 1

供应商 ： 上海信裕

• Tri-Methyl-Histone H3-K9免疫沉淀试剂盒详细信息：
• Product Overview

  
  The EpiQuik™ Tri-Methyl-Histone H3-K9 ChIP Kit is a convenient package of tools that allows the experimenter to investigate interactions of histone H3-K9 tri-methylation and DNA in vivo efficiently. The entire procedure can be completed within 5 hours and produces far superior results than any competitor kits. The EpiQuik™ Tri-Methyl-Histone H3-K9 ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot as well as DNA microarray.
  
  WHY CHOOSE THE EPIQUIK™ TRI-METHYL-HISTONE H3-K9 ChIP KIT?
  

  • The fastest procedure available, which can be finished within 5 hours.
  • Strip microplate format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included: save time and reduce labor.
  • Compatible with all DNA amplification-based approaches.
  • Simple, reliable, and consistent assay conditions.
  Tri-Methyl-Histone H3-K9免疫沉淀试剂盒Principle & Procedure

  
  The EpiQuik™ Tri-Methyl-Histone H3-K9 ChIP Kit contains all reagents required for carrying out a successful chromatin immunoprecipitation for tri-methyl-histone H3-K9 from mammalian cells. Particularly, this kit includes a ChIP-grade tri-methyl-histone H3-K9 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared, and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured tri-methyl-histone H3-K9 protein-DNA complex, reversed and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.
  

  SCHEMATIC PROCEDURE:

  Tri-Methyl-Histone H3-K9免疫沉淀试剂盒Product Components

  
  CP1 (wash buffer)
  CP2 (antibody buffer)
  CP3A (pre-lysis buffer)
  CP3B (lysis buffer)
  CP4 (CHIP dilution buffer)
  CP5 (DNA release buffer)
  CP6 (reverse buffer)
  CP7 (binding buffer)
  CP8 (elution buffer)
  Protease inhibitor cocktail (100x)*
  Proteinase K (10 mg/ml)*
  Normal mouse IgG (0.5 mg/ml)*
  Anti-tri-methyl-H3-K9 (1 mg/ml)*
  8-well assay strips (with frame)
  8-well strip caps
  F-spin column
  F-collection tube
  User guide
  xy115-1 牛 IgG
  xy115-2 牛 IgG
  xy115-3 牛 IgG
  xy115-4 牛 IgG
  xy116-1 牛 IgG ( 液体-Ph 7.4 PBS )
  xy116-2 牛 IgG ( 液体-Ph 7.5 PBS )
  xy116-3 牛 IgG ( 液体-Ph 7.6 PBS )
  xy117-1 马 IgG
  xy117-2 马 IgG
  xy117-3 马 IgG
  xy118-1 马 IgG( 液体液体-Ph 7.6 PBS )
  xy118-2 马 IgG( 液体液体-Ph 7.7 PBS )
  xy118-3 马 IgG( 液体液体-Ph 7.8 PBS )
  xy119-1 鸡 IgG
  xy119-2 鸡 IgG
  xy120-1 鸡 IgG ( 液体液体-Ph 7.8 PBS )
  xy120-2 鸡 IgG ( 液体液体-Ph 7.9 PBS )
  xy121-1 狗 IgG
  xy121-2 狗 IgG
  xy122-1 人白蛋白
  xy122-2 人白蛋白
  xy123-1 人纤维蛋白原
  xy123-2 人纤维蛋白原
  xy124-1 鸡血清蛋白
  xy124-2 鸡血清蛋白
  xy125-1 人血清IgM（液体）70-80%纯度
  xy125-2 人血清IgA（液体）70-81%纯度
  xy201-1 兔抗人IgG
  xy201-2 兔抗人IgA
  xy201-3 兔抗人IgM
  xy202-1 兔抗人纤维蛋白原
  xy203-1 兔抗人血清（多价抗体)
  xy204-1 兔抗人IgG-F(ab）2
  xy205-1 兔抗人白蛋白
  * Spin the solution down to the bottom before use.
  Tri-Methyl-Histone H3-K9免疫沉淀试剂盒User Guide & MSDS