GenMute™ siRNA Transfection Reagent for Smooth Muscle Cells

保存条件 ： 4°C

保质期 ： 1年

英文名 ：GenMute™ siRNA Transfection Reagent for Smooth Muscle Cells

库存 ： 现货 大量

供应商 ： 美中清水湾生物科技有限公司

规格 ：毫升

Description:
GenMute™ siRNA Transfection Reagent for SMC is pre-optimized for transfecting siRNA to smooth muscle cells (SMC).

Refer to the following optimal transfection conditions for maximal silencing on SMC. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.

Summary of Optimal Transfection Conditions: Cell culture medium Confluency of cell on the day of transfection Optimal siRNA concentration Diluent for siRNA and GenMute™ Reagent Incubation Time to Form GenMute™/siRNA Complex Maximal Silencing Efficiency Transfection Results: Reporter Gene Silencing Efficiency (% )

DMEM with 4.5 g/L glucose, 10% FBS ~60% 40 nM 1xGenMute™ Transfection Buffer ~15 minutes at RT 48 hours Renilla Luciferase 85%

Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Data Sheet & Protocol.pdf