抗体名 ：兔抗人程序细胞死亡蛋白 6多克隆抗体

抗体英文名 ：Rabbit anti-human Programmed cell death protein 6 polyclonal Antibody

克隆性 ：多克隆

保存条件 ： -20℃

形态 ： 液体

规格 ：100μg

• 产品类型:
  Primary Antibody>Polyclonal Antibody
• 产品名称:

  兔抗人程序细胞死亡蛋白 6多克隆抗体

• 英文名称:

  Rabbit anti-human Programmed cell death protein 6 polyclonal Antibody

• 兔抗人程序细胞死亡蛋白 6多克隆抗体
• 免疫原:
  Recombinant human Programmed cell death protein 6 protein
• 宿主:
  Rabbit
• 种属反应性:
  Human
• 应用范围:
  WB;ELISA
• 背景:
  Calcium-binding protein required for T-cell receptor-, Fas-, and glucocorticoid-induced cell death. May mediate Ca2+-regulated signals along the death pathway By similarity. Calcium-dependent adapter necessary for the association between PDCD6IP and TSG101.
• 克隆类型:
  Polyclonal
• 抗体亚型:
  IgG
• 纯化方式:
  Caprylic Acid Ammonium Sulfate Precipitation purified
• 标记方式:
  Non-conjugated
• 保存缓冲液:
  Preservative: 0.03% Proclin 300
  Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• 产品提供形式:
  Liquid
• 储存条件:
  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
• 参考文献:
  [1] "Complete sequencing and characterization of 21,243 full-length human cDNAs."Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibaha
• 图片:
  Western blot

  

  All lanes : UV excision repair protein RAD23 homolog A antibody at at 2 µg/ml
  
  Lane 1 : EC109 whole cell lysate
  Lane 2 : 293T whole cell lysate

  Secondary
  Goat polyclonal to Rabbit IgG at 1/15000 dilution

  Predicted band size : 21 kDa

  Observed band size: 36kDa

  Additional bands at : 60 kDa.We are unsure as to the identity of this extra band.

  Why is the actual band size different from the predicted?

  Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include...

  路 post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein

  路 post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases

  路 splice variants - alternative splicing may create different sized proteins from the same gene

  路 relative charge - the composition of amino acids (charged vs non-charged)

  multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.
• 说明书:
• Accession No.:
  O75340
• 兔抗人程序细胞死亡蛋白 6多克隆抗体
• 规格:
  100μg
• 研究领域:
  Apoptosis

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