ActiveMax® Recombinant Human Flt-3 Ligand

英文名 ：ActiveMax® Recombinant Human Flt-3 Ligand

库存 ：In Stock

供应商 ：ACROBiosystems

规格 ：50ug

内毒素:
Less than 1.0 EU per μg by the LAL method.

纯度:
>95% as determined by SDS-PAGE.

活性:
The bio-activity was determined by dose-dependent stimulation of the proliferation of human acute myeloid leukemia cell line OCI-AML5. The ED50 < 0.5 ng/mL, corresponding to a specific activity of >2000000 Unit/mg.

产品背景:
FMS-like tyrosine kinase 3 ligand (Flt-3 Ligand) is also known as FL, Flt3L and FLT3LG, is an α-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. FLT3LG is expressed as a noncovalentlylinked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O- linked carbohydrates. FLT3LG is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1). FLT3LG acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It also induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3LG induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. FLT3LG cooperates with IL2, IL6, IL7, and IL15 to induce NK cell development and with IL3, IL7 and IL11 to induce terminal B cell maturation. Animal studies also show FLT3LG to reduce the severity of experimentally induced allergic inflammation. FLT3LG is crucial for steady-state pDC and cDC development. A lack of FLT3L results in low levels of DCs.

产品描述:
ActiveMax® Human Flt-3 Ligand contains no "tag", and has a calculated MW of 18.9 kDa. The predicted N-terminus is Thr 27 . The reducing (R) protein migrates as 25-30 kDa in SDS-PAGE.