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库存 ：发货周期：现货
供应商 ：博士德生物
应用 ：cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
样本 ：Mouse
规格 ：96T
产品概况
| 货号 | EK1206 |
|---|---|
| 产品名称 | Mouse TSLP ELISA Kit |
| 产品描述 | For quantitative detection of mouse TSLP in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| 免疫原 | NSO, Y20-E140 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 15.6pg/ml-1000pg/ml |
| 基因名称 | TSLP |
| 基因全名 | thymic stromal lymphopoietin |
| Uniprot ID | Q9JIE6 |
| 指标别名 | Thymic stromal lymphopoietin|Thymic stromal lymphopoietin protein TSLP|Tslp|TSLP protein|TSLP_HUMAN |
| 背景资料 | Thymic stromal lymphopoietin, also called TSLP, is a protein belonging to the cytokine family. This gene is mapped to 5q22.1. It encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2(TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. |
| 显色时间 | 25-30分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1206-CAP | 96-well plate precoated with anti- mouse TSLP antibody | 1 |
| EK1206-ST | Lyophilized recombinant mouse TSLP standard | 10ng/tube×2 |
| EK1206-DA | Biotinylated anti- mouse TSLP antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 34 | 252 | 469 | 33 | 246 | 427 |
| Standard deviation | 1.8 | 15.12 | 36.58 | 1.74 | 15.25 | 38.43 |
| CV(%) | 5.3% | 6% | 7.8% | 5.3% | 6.2% | 9% |
Mouse TSLP ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30分钟)| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.029 | 0.098 | 0.157 | 0.298 | 0.574 | 1.094 | 1.689 | 2.267 |
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入驻年限：17年
